Special Issues
Antec introduced the ROXY as an electrochemical reactor up-front MS. The electrochemically-assisted reduction of disulphide bonds followed by on-line mass spectrometric (MS) detection is presented.
Antec introduced the ROXY as an electrochemical reactor up-front MS. The electrochemically-assisted reduction of disulphide bonds followed by on-line mass spectrometric (MS) detection is presented. The method is based on square-wave potential pulses applied on a new type of proprietary working electrode made from titanium alloy. The method does not use any chemical agents and is purely instrumental, resulting in a fully automated platform for fast assessment and characterization of S–S bonds in proteins and peptides.
Figure 1: A schematic of a disulphide bond reduction: Replacing interfering chemicals (for example, DTT, TCEP) by an electrochemical reactor cell used on-line with LC–MS.
An electrochemical reactor offers an advantage over chemical reactions. By changing the applied potential, the extent of disulphide bond cleavage is controlled. More negative potentials result in a shift of the charge state distribution, thereby indicating increased disulphide bond cleavage and unfolding of the protein.
Figure 2: Partial and full reduction of disulphide bonds in α-lactalbumin. The overlapping isotopic pattern of the +9 ion measured with the EC reactor cell turned OFF (top), and ON at E1 = -1000 mV and E1 = -1300 mV.
For a complete application note please visit our website www.myantec.com
Antec
Worldwide: Antec www.myantec.com
USA: Antec USA www.antec-hplc.com
Inside the Laboratory: The Gionfriddo Group at the University at Buffalo
March 28th 2024In this edition of “Inside the Laboratory,” Emanuela Gionfriddo, PhD, an associate professor of chemistry at the University at Buffalo, discusses her group’s current research endeavors, including using solid-phase microextraction (SPME) coupled to liquid chromatography (LC) and gas chromatography (GC) to further understand the chemical relationship between environmental exposure and disease and elucidate micropollutants fate in the environment and biological systems.
Transferring Methods to Compact and Portable HPLC
February 14th 2024The current trend in laboratory equipment design is the miniaturization of laboratory instruments. Smaller-scale HPLC instruments offer benefits that cannot be matched by analytical-scale equipment, especially in the areas of portability, reduced fluid volumes, and reduced operating costs. Yet, the miniaturization of laboratory equipment has brought with it a unique set of challenges, including transferring methods to compact LC. Capillary LC expands the use of LC to applications not currently done using conventional LC in a wide array of application areas, including pharmaceutical, food and beverage, petrochemical, environmental, and oil and gas. Greg Ward, Axcend’s CEO wrote, “Customers want an HPLC system with a small footprint, low flow rates and green chemistry.” Join his podcast where he shares method transfer in these application areas.