Determination of Adenine and Guanine Nucleotides in Tissue Extracts

Hamilton Company

The Application Notebook

The Application Notebook, The Application Notebook-09-02-2017, Volume 35, Issue 9

Adenosine triphosphate (ATP) is the universal energy currency in biological systems. ATP is synthesized from adenosine mono and di-phosphate substrates. The relative ratio of ATP, ADP, and AMP is an important indicator of metabolic standing: the energy charge

is considered a quantitative measure of energy status, and serves as a barometer of sorts, as aberrant change in EC is associated with onset of numerous pathological states. Measurement of adenosine nucleotides, therefore, represents an important and insightful diagnostic.

In the present study, an ion pair reversed-phase HPLC method was developed to separate ATP, ADP, and AMP from like-phosphorylated guanosine metabolic products (such as GTP, GDP, GMP) enabling quantification of these nucleotides from tissue extracts.

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