Paper spray-mass spectrometry vs. liquid chromtography-tandem mass spectrometry (LC-MS/MS): Can rapid screening rival the gold standard in ER drug testing?
|Articles|December 5, 2012
- The Column-12-03-2012
- Volume 8
- Issue 22
Determination of Histamine in Human Serum/Plasma by SPE and HILIC LC-MS-MS
Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.
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Histamine is a low-molecular-weight amine that plays several critical physiological roles, most notably in mediating the inflammation response. Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression. Sample concentration and cleanup with solid phase extraction (SPE) further enhances the process and significantly increases throughput.
Articles in this issue
almost 14 years ago
A Day at the Racesalmost 14 years ago
Market Profile: The Analytical and Life Science Instrumentation Industryalmost 14 years ago
Sweet potentialalmost 14 years ago
Instrumental Innovationsalmost 14 years ago
Healthy hamalmost 14 years ago
GM seeds to save salmon?almost 14 years ago
Tips & Tricks: GPC/SEC Method Developmentalmost 14 years ago
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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) finds high fentanyl levels do not predict toxicity in tolerant users.
Gas chromatography-ion mobility spectrometry (GC-IMS) identifies odor markers that flag early mold growth in cigar tobacco.
Lorna Nisbet from the Leverhulme Research Centre for Forensic Science, discusses a GC–MS. technique developed to detect anabolic-androgenic steroids in e-cigarettes seized from Scottish prisons.
Kinjal Bhatt and Ina Varfaj trace their pull toward analytical science to a saturated chromatography peak and the moment their first chiral peaks separated.
Gérard Hopfgartner explains why pairing SFC with dopant-assisted APPI can match, or even beat, electrospray sensitivity, even at microflow LC rates.
Emma Macturk from LECO Corporation explains which forensic applications benefit from GC×GC’s greater peak capacity, and when the additional separation power may only provide limited benefits.
Koji Yamaguchi of the International University of Health and Welfare (Narita, Japan) discusses a novel derivatized liquid chromatography-tandem mass spectrometry (LC-MS/MS) method plus propofol glucuronide (PRG) marker which improves forensic propofol detection.
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