Gas chromatography–mass spectrometry (GC–MS) and ion chromatography profile wasabi and mustard heat and acidity.
|Articles|December 5, 2012
- The Column-12-03-2012
- Volume 8
- Issue 22
Determination of Histamine in Human Serum/Plasma by SPE and HILIC LC-MS-MS
Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.
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Histamine is a low-molecular-weight amine that plays several critical physiological roles, most notably in mediating the inflammation response. Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression. Sample concentration and cleanup with solid phase extraction (SPE) further enhances the process and significantly increases throughput.
Articles in this issue
almost 14 years ago
A Day at the Racesalmost 14 years ago
Market Profile: The Analytical and Life Science Instrumentation Industryalmost 14 years ago
Sweet potentialalmost 14 years ago
Instrumental Innovationsalmost 14 years ago
Healthy hamalmost 14 years ago
GM seeds to save salmon?almost 14 years ago
Tips & Tricks: GPC/SEC Method Developmentalmost 14 years ago
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