Jeff Layne

Ion-pairing chromatography has traditionally been implemented with the addition of an ion-pairing reagent into the mobile phase. Here, an alternative method is presented, in which the ion-pairing reagent is deposited on column as a sample additive instead of in the mobile phase.

Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.

Urinary excretion profiling is a key element in the pharmokinetic characterization of new chemical entities (NCEs). Recent advances in ultra-high performance liquid chromatography (UHPLC) column technology offer improved results for liquid chromatography tandem mass spectrometry (LC–MS¬–MS) analysis, enabling rapid quantification of specific drugs and their metabolites in biological matrices.

Phenomenex

Heterocyclic amines (HCAs) are a class of compounds that are formed when amino acids from meat proteins react with glucose and creatine under high-temperatures, as is associated with frying or grilling over an open flame (1–3). Studies have correlated the consumption of high levels of HCAs with an increased risk of some forms of cancer.

The current state of economic affairs is tumultous; as the economy struggles to bounce back, companies across all industries are becoming more creative in finding ways to reduce costs without sacrificing the quality of their goods or services. According to a recent survey conducted by the Society of Forensic Toxicologists (SOFT), 64.5% of forensic labs surveyed are implementing changes, or plan to implement changes, as a result of limited resources.

Phenomenex Application Note

A new 3.6 µm 100 Å HPLC–UHPLC column (Aeris PEPTIDE) has been introduced that is specifically designed to improve separations of peptide and peptide mapping applications.

A new widepore core-shell HPLC–UHPLC column (aeris WIDEPORE) has been introduced that is specifically designed to improve protein separations.

Phenomenex Application Note

A method is illustrated for the simultaneous analysis of ethyl glucuronide and ethyl sulfate in human urine samples.

This study demonstrates a faster method of analysis when coupling a simplified and effective SPE procedure with a core-shell HPLC column and LC–MS-MS detection.

Barbiturates are a class of antidepressants whose abuse and addiction by recreational users has become a widespread problem (www.nlm.nih.gov). In our work we strived to streamline the barbiturate screening process to provide a fast, cost-effective, and reproducible method from start to finish for forensic labs who are involved in high-throughput processing.

The chiral analysis and purification of proton pump inhibitors (PPIs) has become a popular topic as more of these drugs fall out of patent protection each year. Due to their wide range of enantiomeric selectivity, a set of polysaccharide-based chiral stationary phases (CSPs) was screened to identify methods for the successful enantioseparation of four benzimidazoles; rabeprazole, lansoprazole, omeprazole, and pantoprazole.

This study evaluates the performance of a C18 core-shell phase that incorporates a C18 ligand with iso-butyl side chains. The Kinetex? XB-C18 HPLC/ UHPLC column delivers a fast and effective separation of several common preservatives in cosmetics.

Testosterone was extracted from human serum by strong anion exchange polymeric SPE and analyzed using a Kinetex C18, 30 ? 2.1 mm, 1.7 ?m column and positive polarity ESI LC–MS-MS system. Kinetex sub-2 ?m core-shell technology offers higher efficiencies than traditional sub-2 ?m columns, producing greater chromatographic resolution, sensitivity, and higher peak capacities.

Recently a newly developed Kinetex 2.6 µm core-shell chromatographic particle has been commercialized that offers the performance benefits of sub-2 µm fully-porous particles but at substantially lower operating pressures.