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The complexity of method development for GPC/SEC is often underestimated. Although only isocratic separation is applied, the development of a robust and stable method can be challenging. It is important that measures are taken to develop a suitable method that will deliver long-term reproducible results, especially when biomolecules, ions or functional groups are present.
The complexity of method development for GPC/SEC is often underestimated. Although only isocratic separation is applied, the development of a robust and stable method can be challenging. It is important that measures are taken to develop a suitable method that will deliver long‑term reproducible results, especially when biomolecules, ions or functional groups are present.