Embracing HILIC: Dispelling Myths and Realizing the Benefits


Webinar Date/Time: Thu, Dec 8, 2022 2:00 PM EST

Discover the advantages of HILIC as an option for very polar and hydrophilic compounds and learn about the HILIC stationary phases available on Fused-Core® particles.

Register Free: https://www.chromatographyonline.com/lcgc_w/embrace_hilic

Event Overview:

The mention of moving from the beloved reversed-phase separation mode to HILIC can ignite anxiety in many a method development scientist. But what do you do when many polar compounds are not well retained under reversed-phase conditions? Utilizing Hydrophilic Interaction Liquid Chromatography (HILIC) as a mode of chromatography is very beneficial for separations involving very polar analytes. However, some labs consider HILIC as a last resort method and would prefer to avoid its use. This webinar aims to dispel the myths around HILIC and provide options for those struggling with methods for polar analytes, such as metabolites, basic drugs, and carbohydrates.

Key Learning Objectives:

  • Understand the advantages of using HILIC for polar analytes
  • Learn tips and tricks to successfully develop HILIC methods
  • Review example HILIC applications


Stephanie A. Schuster, Ph.D.
Senior Technical Support Scientist
Advanced Materials Technology, Inc.

Dr. Stephanie A. Schuster earned her Bachelor of Arts degree in Chemistry and Biochemistry in 1998 at La Salle University, where she graduated cum Laude from the Honors Program. She earned her Ph.D. in 2007 under the guidance of Professor Joe P. Foley at Drexel University. Her doctoral dissertation focused on the use of vesicles as pseudostationary phases in capillary electrophoresis. This system was investigated as a potential in vitro model for intestinal permeability.

After earning her Ph.D. at Drexel, Dr. Schuster joined Atlantic Diagnostic Laboratories (ADL), a combined clinical and forensic testing laboratory. While at ADL, she authored a procedure manual, and co-authored or contributed to other documentation, in preparation for College of American Pathology (CAP) inspection. She was instrumental in implementing general laboratory improvements (appropriate labeling, daily maintenance logs, etc.) in accordance with CAP guidelines. Dr. Schuster also assisted with method development using an Applied Biosystems 3200 Q TRAP LC/MS/MS System.

Beginning in June of 2009, Dr. Schuster joined Advanced Materials Technology, Inc. (AMT) located in Wilmington, DE as a research scientist working with Dr. Jack Kirkland, providing contributions to the commercial development of products specifically designed for the separation of peptides and proteins. During the summer of 2015, Dr. Schuster began to transition away from research and development activities and more toward applications development and quality assurance. She is currently a Senior Technical Support Scientist at AMT. As such, Dr. Schuster works closely with AMT’s worldwide network of distributors and assists customers with optimizing and trouble-shooting their applications. Her primary interests are separations and novel materials development. Dr. Schuster has several peer-reviewed articles and is currently a member of the Chromatography Forum of the Delaware Valley.

Times and Dates:

Thu, Dec 8, 2022 2:00 PM EST


Advanced Materials Technology

Register Free: https://www.chromatographyonline.com/lcgc_w/embrace_hilic