Estradiol Extraction From Serum Using SLE+

This application note describes the use of ISOLUTE SLE+ for extraction of estrone (E1) and estradiol (E2) from human serum. Analysis is performed by an HPLC-MS/MS method that gives limits of detection of 0.001 ng/mL without derivatization or fluoride adduct stabilization. ISOLUTE SLE+ products provides high analyte recoveries, no emulsion formation and significantly reduced sample preparation time compared to traditional liquid-liquid extraction.