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American and European Foulbrood in honeybees is caused by two bacterial species, Paenibacillus larvae and Melissococcus pluton, respectively. For control of these bacteria, only a few antibiotics are recommended in Europe and the U.S.A. However, a variety of antibacterial agents are used, the sulfa drugs being the most popular. Residues of these antibiotics are quite often found in honey samples and are of concern to consumers around the world due to toxic or allergic reactions. Earlier sample preparation methods involved using silica-based C18 or neutral polymers as sorbents with an aqueous wash prior to elution of sulfonamides. In this communication, we describe a simple and effective method for cleanup and quantitation of antibacterial sulfonamides from honey using the polymeric strong cation exchange SPE sorbent strata-X-C and a Gemini HPLC column.