Fluorinated Monolithic Columns for LC
Researchers from Queen's University (Kingston, Ontario, Canada) developed porous polymer capillary columns with varied flurous content for chromatography of tagged analytes.
Researchers from Queen's University (Kingston, Ontario, Canada) developed porous polymer capillary columns with varied flurous content for chromatography of tagged analytes. They used a UV-initiated radical polymerization process to create the monolithic columns and tested them using flow-induced back pressure and scanning electron microscopy. The columns were able to separate fluorous-tagged analytes under gradient conditions, and the researchers found that the fluorinated monolithic columns exhibited greater selectivity for fluorous analytes than did comparable nonfluorinated monolithic columns.
Quantifying Isavuconazole in Dried Blood Spots Using HPLC
May 21st 2025Isavuconazole, an antimycotic agent used to treat fungal infections, can typically be found during dried blood spot sampling. However, there are obstacles that keep it from being an ideal approach for properly determining the drug’s presence.
Reversed-Phases for LC Deliberately Doped with Positive Charge: Tips and Tricks for Effective Use
May 13th 2025In this month's edition of LC Troubleshooting, Dwight Stoll and his fellow researchers discuss both the benefits (improved peak shape/loading) and challenges (excessive interaction) associated with charge-doped reversed-phase (RP) columns for both analytical and preparative separations.
