Gerald Drager and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
Gerald Drger and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
The system uses immobilised Ni-NTA (nitrilotriacetic acid) with a new tyrosine linker, attached to polymeric materials inside the reactor chamber. The reactor is integrated into an HPLC set-up. The NTA component is used in metal ion affinity chromatography, which in turn can be used to purify His6-tagged proteins from crude cell extracts. The polymeric materials in the reactor have also been altered to be more polar, ideal for biological molecules.
The Chromatographic Society 2025 Martin and Jubilee Award Winners
December 6th 2024The Chromatographic Society (ChromSoc) has announced the winners of the Martin Medal and the Silver Jubilee Medal for 2025. Professor Bogusław Buszewski of Nicolaus Copernicus University in Torun, Poland, has been awarded the prestigious Martin Medal, and the 2025 Silver Jubilee Medal has been awarded to Elia Psillakis of the Technical University of Crete in Greece.
RAFA 2024: Giorgia Purcaro on Multidimensional GC for Mineral Oil Hydrocarbon Analysis
November 27th 2024Giorgia Purcaro from the University of Liège was interviewed at RAFA 2024 by LCGC International on the benefits of modern multidimensional GC methods to analyze mineral oil aromatic hydrocarbons (MOAH) and mineral oil saturated hydrocarbons (MOSH).