Gerald Drager and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
Gerald Drger and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
The system uses immobilised Ni-NTA (nitrilotriacetic acid) with a new tyrosine linker, attached to polymeric materials inside the reactor chamber. The reactor is integrated into an HPLC set-up. The NTA component is used in metal ion affinity chromatography, which in turn can be used to purify His6-tagged proteins from crude cell extracts. The polymeric materials in the reactor have also been altered to be more polar, ideal for biological molecules.
Microseparations and Analysis Covered at HPLC 2024
July 23rd 2024At HPLC 2024 in Denver, Colorado, various sessions will be led by experienced scientists, covering different aspects of chromatography techniques and maximizing their effectiveness. Here is some information about tomorrow’s sessions.
Automated LC Method Development: An HTC-18 Interview with Bob Pirok
July 23rd 2024At HTC-18 in Leuven, Executive Editor of LCGC International, Alasdair Matheson, spoke to this year’s winner of the 2024 HTC-18 Innovation Award, Bob Pirok from the University of Amsterdam, about his innovative research on automated LC method development.