Gerald Drager and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
Gerald Drger and colleagues from University of Hannover in Leibniz, Germany, have developed a system that enables immobilization and purification of enzymes in the same reactor, which can then be used for a variety of enzymatic syntheses. Using this technique it is possible to obtain ready-to-use enzyme reactors from crude protein mixtures within minutes.
The system uses immobilised Ni-NTA (nitrilotriacetic acid) with a new tyrosine linker, attached to polymeric materials inside the reactor chamber. The reactor is integrated into an HPLC set-up. The NTA component is used in metal ion affinity chromatography, which in turn can be used to purify His6-tagged proteins from crude cell extracts. The polymeric materials in the reactor have also been altered to be more polar, ideal for biological molecules.
Best of the Week: What’s New in MS, 2024 Young Chemist Award Winner
March 22nd 2024This week, LCGC International published a variety of articles on the hottest topics in chromatography and beyond. Below, we’ve highlighted some of the most popular articles, according to our readers. Happy reading!
Inside the Laboratory: The Schug Group at the University of Texas at Arlington
March 22nd 2024In this edition of “Inside the Laboratory,” Kevin Schug, PhD, a full professor of chemistry at the University of Texas at Arlington, discusses his laboratory’s group work in environmental monitoring around water and soil quality near oil and gas extraction, using techniques such as liquid chromatography (LC), gas chromatography (GC), supercritical fluid chromatography (SFC), and coupling these techniques with mass spectrometry (MS).
Cloruson and Related Substances Studied Using Original Ion-Pair UHPLC Method
March 19th 2024In a recent study out of Boehringer Ingelheim Animal Health USA Inc. in North Brunswick, New Jersey, scientists investigated cloruson and its related substances using an original ion-paired reversed phase ultraperformance liquid chromatography (IP-UHPLC) method.