HPLC-UV Method for the Determination of Telmisartan Using a Core Enhanced Technology Accucore Column
The Application Notebook
This application note demonstrates the use of the Thermo Scientific Accucore RP-MS column for the determination of telmisartan by HPLC-UV.
This application note demonstrates the use of the Thermo Scientific Accucore RP-MS column for the determination oftelmisartan by HPLC-UV.
Accucore™ HPLC columns use Core Enhanced Technology™ to facilitate fast and high efficiency separations. The 2.6 µm diameter particles are not totally porous, but rather have a solid core and a porous outer layer. The optimized phase bonding creates a series of high coverage, robust phases. Accucore RP-MS uses an optimized alkyl chain length for more effective coverage of the silica surface. This coverage results in a significant reduction in secondary interactions and thus highly efficient peaks with very low tailing. The tightly controlled 2.6 µm diameter of Accucore particles results in much lower backpressures than typically seen with sub-2 µm materials.
Telmisartan is an angiotension II receptor antagonist used for the treatment of hypertension.
This application note demonstrates the successful analysis of telmisartan using an Accucore RP-MS column.
Sample Preparation
Working standard contained 20 µg/mL of telmisartan in water.
Thermo Scientific Column
Accucore RP-MS 2.6 µm, 50 × 2.1 mm (P/N 17626-052130)
Thermo Scientific Accela UHPLC system
Flow rate: 0.4 mL/min
Column temperature: 25 °C
Injection details: 1 µL partial loop
Injection wash solvent: water
UV detector wavelength: 230 nm
Backpressure: 150 bar
Mobile Phase
70:30 (v/v) 20 mM ammonium acetate/acetonitrile
Consumables
Fisher Scientific HPLC grade water (P/N W/0106/17)
Fisher Scientific HPLC grade ammonium acetate (P/N A/3446/50)
Fisher Scientific HPLC grade acetonitrile (P/N A/0626/17)
NSC Mass Spec Certified 2 mL clear vial with blue bonded PTFE silicone cap (P/N MSCERT4000-34W)
Telmisartan purchased from Sigma Aldrich
Figure 1: Chromatogram of telmisartan analyzed using an Accucore RP-MS 2.6 µm, 50 à 2.1 mm column.
Results
The analysis was performed on an Accucore RP-MS 2.6 µm, 50 × 2.1 mm column. As shown in Figure 1, telmisartan was analyzed in less than 2 min. Table I shows the results from six replicate injections.
Table I: Retention time and asymmetry results for telmisartan
Conclusions
Replicate injections of telmisartan showed that Accucore RP-MS produced stable and reproducible results. This demonstrates that Accucore RP-MS is an excellent choice of column for the rapid analysis of telmisartan.
Thermo Fisher Scientific
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tel: +44 (0) 1928 534110
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