Improving Separation Efficiency with CORTECS™ Premier Columns that Feature Solid-Core Particles and MaxPeak™ Premier HPS Technology
High efficiency columns increase resolution by reducing peak widths. This enables easier peak integration and identification, as the peaks of interest are better separated from each other and from potential background or excipient peaks. There are several ways for an analyst to improve separation efficiency; one being to use columns packed with smaller particle size stationary phases. Another way is to use longer columns. However, a drawback in the use of both options is that they can be limited by the system operating pressure. Another path forward is the use of solid-core or superficially porous particles (SPP), which have been proven to improve efficiency without sacrificing operating pressure. This app note shows incremental steps on how to improve separation efficiency for a mixture of three analytes.
Pick Your Poison. Isolation of Paclitaxel (Mar 2025)
March 7th 2025The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.
