Iridoid detection in rat plasma

Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography–mass spectrometry (LC–MS) method for the simultaneous determination of geniposide 6α-hydroxygeniposide and genipin gentiobioside in rat plasma.

After the addition of internal standard salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a C18 column (200 mm × 4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2–250 ng/mL for both 6α-hydroxygeniposide and genipin gentiobioside and 2–2000 ng/mL for geniposide, respectively.

The validated method was successfully applied to a pharmacokinetics study of geniposide, 6α-hydroxygeniposide and genipin gentiobioside in rats after oral administration of a Zhi-zi-chi (gardenia and prepared soybean) decoction.

The abstract was published in the October 2011 issue of Journal of Separation Science, Volume 34, pp 2854–2860.

This story originally appeared in The Column. Click here to view that issue.