The Column, The Column-11-07-2011, Volume 7, Issue 20
Automating the DNA sequence process is made possible by the use of fluorescent tags. With fluorescent dyes, the entire experiment can be performed in a single solution instead of four different ones. Using Sanger-based methods, the DNA fragments migrate through the gel and the colour associated with each successive peak is detected using a laser. The DNA sequence is read by determining the sequence of colours in the peaks as they pass the detector and then fed directly to a computer for instant results.
Automating the DNA sequence process is made possible by the use of fluorescent tags. With fluorescent dyes, the entire experiment can be performed in a single solution instead of four different ones. Using Sanger-based methods, the DNA fragments migrate through the gel and the colour associated with each successive peak is detected using a laser. The DNA sequence is read by determining the sequence of colours in the peaks as they pass the detector and then fed directly to a computer for instant results.
