Automated DNA Sequencers
Automating the DNA sequence process is made possible by the use of fluorescent tags. With fluorescent dyes, the entire experiment can be performed in a single solution instead of four different ones. Using Sanger-based methods, the DNA fragments migrate through the gel and the colour associated with each successive peak is detected using a laser. The DNA sequence is read by determining the sequence of colours in the peaks as they pass the detector and then fed directly to a computer for instant results.
Automating the DNA sequence process is made possible by the use of fluorescent tags. With fluorescent dyes, the entire experiment can be performed in a single solution instead of four different ones. Using Sanger-based methods, the DNA fragments migrate through the gel and the colour associated with each successive peak is detected using a laser. The DNA sequence is read by determining the sequence of colours in the peaks as they pass the detector and then fed directly to a computer for instant results.
Investigating Plastic Contamination in Olive Oil with GC–MS/MS
January 17th 2025The objective of this study was to investigate contamination by 32 plasticizers in olive oil throughout its production and packaging process. Separation and detection were carried out by gas chromatography tandem mass spectrometry (GC–MS/MS), without the need for pre-concentration steps.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Top Execs from Agilent, Waters, and Bruker Take the Stage at J.P. Morgan Healthcare Conference
January 16th 2025The 43rd Annual Healthcare J.P. Morgan Healthcare Conference kicked off in San Francisco earlier this week. Here’s what top executives from Agilent, Bruker, and Waters, discussed during the event.
Measuring Lavender Essential Oil’s Effect on Cancerous Tumors with GC-MS
January 15th 2025A recent study aimed to investigate the impact of lavender essential oil (LEO) and of its terpenic components on the properties of glioblastoma (GBM) cells in an in vitro model. The study demonstrated that LEO induces a proliferation slowdown and an impairment of cell migration of GBM cells besides reducing oxidative stress.
