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In this fourth part of " Conversations with Michael Dong," a series on fundamentals of HPLC in pharmaceutical analysis, LCGC International’s "Perspectives in HPLC" column editor explains why buffers are most often used in Mobile Phase A in high-pressure liquid chromatography of pharmaceuticals.

Skipping solid-phase extraction (SPE) cleanup rarely hurt liquid chromatography–tandem mass spectrometry (LC–MS/MS) plant hormone results in tomato.

The panel considers how AI and automation could reshape peptide method development, and why scientific judgment will remain essential.

Gas chromatography–mass spectrometry (GC–MS) and docking reveal A. excelsa leaf compounds that may help in cancer treatment.

Gert Desmet offers his insights on the potential benefits AI/ML could offer the separation science community in the future.

Quantitative non-targeted screening with predicted response factors is not for regulation, but can rank environmental chemicals by concentration and toxicity.

Gert Desmet explores the current role of AI/ML in separation science.

Gas chromatography–mass spectrometry (GC–MS) reveals a four-stage polycyclic aromatic hydrocarbons (PAH) breakdown in oil sludge co-composted with manure.