LCGC Columnist Receives Medal
Ronald E. Majors, a senior chemist at Agilent and LCGC's "Column Watch" and "Sample Preperation Perspectives" columnist, received the Chromatographic Society's 2007 Martin Gold Medal at HPLC 2008.
Ronald E. Majors, a senior chemist at Agilent and LCGC's "Column Watch" and "Sample Preperation Perspectives" columnist, received the Chromatographic Society's 2007 Martin Gold Medal at HPLC 2008. The award, named after the Nobel Prize winner A.J.P. Martin, was presented by Professor W. John Lough, president of the Chromatographic Society of the United Kingdom.
Majors has contributed significantly to separation science research through the years, including his work in HPLC column technology, particle size studies, packing methodologies, and chemically bonded phases. He was the first to efficiently pack 5-μm particles into high performance columns and was responsible for the introduction of the first commercial microparticulate column.
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
