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In a new study published in the Journal of the American Society for Mass Spectrometry, a new method has been developed for site-specific fluorescent labeling of hemagglutinin-specific antigen binding fragments (1). This new method uses amine chemistry and mass spectrometry to precisely label the antigen binding fragment at specific locations, allowing for more accurate detection and analysis of the protein.
Hemagglutinin is a protein found on the surface of influenza viruses, and is a target for many influenza vaccines. The antigen binding fragment is a small part of an antibody that specifically binds to the hemagglutinin protein. By labeling the antigen binding fragment with a fluorescent molecule, researchers can more accurately detect and analyze the protein, which can help in the development of new vaccines and treatments for influenza.
The new method developed in this study uses amine chemistry to label the antigen binding fragment at specific locations. The researchers then used mass spectrometry to confirm the precise location of the label, ensuring that the protein remained intact and functional.
One of the advantages of this new method is that it allows for the precise labeling of the antigen binding fragment, which can help in the development of more accurate and effective vaccines and treatments for influenza. It also provides a valuable tool for the analysis of other proteins and biomolecules.
This study provides a promising new method for the site-specific fluorescent labeling of hemagglutinin-specific antigen binding fragments, and may lead to new and important applications in the development of new vaccines and treatments for influenza.
(1) Zou, G.; Ivleva, V. B.; Wolff, J. J.; Yang, R. S.; Alabanza, C.; Barefoot, N.; Cai, C.; Yang, Y.; Gowetski, D. B.; Gall, J. G.; Lei, Q. P. Site-Specific Fluorescent Labeling of Hemagglutinin-Specific Antigen Binding Fragment through Amine Chemistry Revealed by Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2023, March 31. DOI:10.1021/jasms.2c00330