Mobile Phase Optmization Strategies for Reversed Phase HPLC



Webinar Date/Time: Thu, Feb 15, 2024 11:00 AM EST

Unlock the secrets to efficient method development in our upcoming webcast! Delve into the intricacies of mobile phase composition, exploring topics like reversed phase retention mechanisms, solvent selection dilemmas, eluotropic strength shortcuts, and much more. Join us as we unravel the science behind achieving optimal resolution in record time – a must for every chromatographer.

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Event Overview:

This webcast will look at strategies for optimizing mobile phase composition in order to achieve the required resolution in the minimum amount of time. This is, of course, the primary goal of everyone undertaking method development. The chemistry of the aqueous and organic portions of the mobile phase will be studied, and we will discuss how to use these components to affect retention and improve the selectivity and resolution within our separations.

Topics Covered:

  • Review of reversed phase retention mechanisms
  • Methanol or acetonitrile - which one is best?
  • Eluotropic strength - quick ways to reach the ideal
  • Changing solvent - useful tools and approaches
  • Which buffer, what strength?
  • Mobile phase pH - understand the effects
  • Optimizing pH vs. retention
  • Strategies for when all else fails


Kevin Schug
Professor and the Shimadzu Distinguished Professor of Analytical Chemistry, Department of Chemistry and Biochemistry
The University of Texas at Arlington

Kevin A. Schug is Professor and the Shimadzu Distinguished Professor of Analytical Chemistry in the Department of Chemistry and Biochemistry at The University of Texas at Arlington (UTA). He is also Director of the Collaborative Laboratories for Environmental Analysis and Remediation (CLEAR) at UTA. He received his B.S. degree in Chemistry in 1998 from the College of William and Mary, and his Ph.D. degree in Chemistry from Virginia Tech in 2002 under the direction of Prof. Harold M. McNair. From 2003-2005, he performed post-doctoral research at the University of Vienna in Austria with Prof. Dr. Wolfgang Lindner.

Since joining UTA in 2005, his research has been focused on the theory and application of separation science and mass spectrometry for solving a variety of analytical and physical chemistry problems, in the fields of environmental, pharmaceutical, biological, and energy research. He has over 200 peer-reviewed publications and over 600 presentations, posters, and invited talks to his group’s credit. He has been the primary mentor and research advisor to more than 30 graduate and 80 undergraduate students. Dr. Schug has received several research awards, including the 2009 Emerging Leader Award in Chromatography by LCGC Magazine and the 2013 American Chemical Society Division of Analytical Chemistry Young Investigator in Separation Science Award. He was named to the 2019 and the 2021 The Analytical Scientist’s Top 100 Power List of the most influential analytical chemists in the world. For his teaching, he received the 2014 University of Texas System Regents’ Outstanding Teaching Award and in 2017, was awarded the J. Calvin Giddings Award for Excellence in Analytical Chemistry Education by the American Chemical Society. He is a Fellow of both the University of Texas System’s and U.T. Arlington’s Academy of Distinguished Teachers.

Tony Taylor
Chief Scientific Officer
Element Life Sciences EMEAA, Element Materials Technology

Tony Taylor is the chief scientific officer of Element Life Sciences EMEAA. He has worked in the pharmaceutical, polymer, contract analysis, and consulting industries for more than 35 years. He is an experienced chromatographer who isn’t afraid to admit that every day is a school day in the lab. He is a founder of CHROMacademy, the website where chromatographers to go learn.

He has taught thousands of chromatographers globally and his natural enthusiasm for analytical science makes him a very popular speaker on webcasts, podcasts, and webinars.

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