Molecularly Imprinted SPME in Analysis of Beta Blockers

Researchers from Sun Yat-sen University and South China Normal University (both in Guangzhou, China) developed a sample preparation method for extracting the beta-blockers propranolol, atenolol, pindolol, and alprenolol from human plasma and urine samples.

Researchers from Sun Yat-sen University and South China Normal University (both in Guangzhou, China) developed a sample preparation method for extracting the beta-blockers propranolol, atenolol, pindolol, and alprenolol from human plasma and urine samples. The method involved preparing a molecularly imprinted polymer (MIP)–coated solid-phase microextraction (SPME) fiber with propranolol as a template. The MIP coating had an average thickness of 25 µm and was highly crosslinked and porous. The MIP-coated fiber provided specific selectivity for the beta-blockers compared with only nonspecific adsorption achieved using non-imprinted polymer fibers. They linked the SPME method with high performance liquid chromatography for a quantitative analysis.