New Method for Selective Extraction of Levoglucosan and Its Isomers from Complex Matrices for Analysis

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A new method that combines LE-SPE and LC-ESI-MS/MS can improve extraction of monosaccharide anhydrides from sediment samples.

A team of researchers from the University of Barcelona have developed a new method for the selective extraction of monosaccharide anhydrides (MAs) from sediment samples, which is crucial for their analysis (1).

MAs, particularly levoglucosan, are often used as biomarkers to determine the sources and evolution of organic matter in sediments. However, their analysis is complicated because of the lack of reliable technologies that can selectively extract these compounds from complex matrices.

The new method takes advantage of the affinity between MAs and immobilized Na+ ions related to ligand-exchange processes (LE-SPE). By using nonaqueous mobile phases such as DCM/MeOH mixtures, the capacity factor of LE-SPE columns is enhanced. The researchers have used this method to selectively extract MAs from lacustrine, coastal, and deep-sea oceanic sediment samples.

The analytical procedure produces extracts with low ion suppression effects, resulting in ideal conditions for MAs quantification with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) systems, regardless of the sedimentary matrix and MAs concentration. The method yields repeatable concentration values and has an instrumental dynamic range of 10–10000 pg injected.

The combination of LE-SPE and LC-ESI-MS/MS has the potential to produce sensitive and reliable technologies to analyze saccharides and amino acids in environmental and biological samples.


The researchers believe that their method will be useful for studies that aim to understand the evolution of organic matter in sedimentary environments. The method will also be valuable in the analysis of other water-soluble non-ionic compounds in complex matrices.

Overall, the new method is a significant advancement in the field of sediment analysis, offering a reliable and efficient way to extract MAs from sediment samples. The team hopes that their findings will pave the way for future studies and applications of the method in environmental and biological research.


(1) Daytian, N.; Penalva, N.; Rosell-Melé, A.; Villanueva, J. Selective extraction of levoglucosan and its isomers from complex matrices using ligand exchange-solid phase extraction for analysis by liquid chromatography-electrospray ionization-tandem mass spectrometry. J. Chromatogr. A. 2023, 1695, 463935. DOI: 10.1016/j.chroma.2023.463935.