The Application Notebook
Volume 36, Issue 12
This application note outlines the performance benefits achieved with UCT’s LipiFiltr® cleanup cartridge for the analysis of pesticides in oil-based cannabis products using LC–MS/MS analysis.
This application note outlines the performance benefits achieved with UCT’s LipiFiltr® cleanup cartridge for the analysis of pesticides in oil-based cannabis products using LC–MS/MS analysis. The pesticides evaluated in this study include those listed in the Oregon monitoring list (≈60 pesticides). Cannabidiol (CBD) oil was used as the representative matrix. A comparison of pre- and post-LipiFiltr® cleanup using full scan GC–MS is also presented to demonstrate the removal of lipid co-extractives, without compromising cannabinoid content.
LC–MS/MS: SCIEX Triple Quad 6500ï« with SCIEX V Method™ for pesticides in cannabis
GC–MS: 30 m × 0.25 mm, 0.25-µm Rxi-5Sil MS column with a Topaz Precision split inlet liner. A 1 µL injection volume with a split ratio of 10:1 was used.
Results and Conclusion
GC–MS (full scan) analysis showed significant removal of lipids and other high-molecular-weight matrix co-extractives using the LipiFiltr® cartridge, with no significant negative effect on CBD. This was backed up by LC–DAD analysis (not detailed here). While further work should be completed on other oil-based products, including those with a high THC content, the results highlighted here demonstrate that the LipiFiltr® cartridge can be successfully used to remove matrix co-extractives from oil-based cannabis samples without compromising CBD content or pesticide recoveries.