Past, Present, and Future of Chiral Separations

March 2, 2021

The talks in this Friday afternoon symposium will cover topics related to column and stationary phase development and instrumentation, along with the analytical and production-scale applications of chiral chromatography.

The session will kick off at 1:30 pm with a talk by Michael Wey and his group at The University of Texas at Arlington and AZYP LLC, on enhancing chiral separations using superficially porous particles. Superficially porous particles (SPPs), also known as core–shell particles, are now commonplace on high performance liquid chromatographs. Wey will discuss how adoption of SPPs have redefined the state of chiral separation technology.

Next, at 2:05 pm, Nelu Grinberg of Grinberg Consulting will discuss vibrational circular dichroism spectroscopy (VCD) for determining supramolecular interactions in enantiomeric separations. Understanding the interactions between the select and the selector occurring during enantiomeric separations makes method development easier. The study of such interactions can be performed using VCD. Grinberg will explain how his group used VCD to facilitate a better understanding of chiral separation and consequently increased the speed and efficiency of the method development process.

Larry Miller of Amgen will then describe, at 2:40 pm, preparative enantioseparations in pharmaceutical research and development (R&D). Over the past 30 years, preparative chiral chromatography has evolved to become a critical technique in pharmaceutical discovery and development. Using relevant pharmaceutical examples, this talk will provide a historical overview of preparative enantioseparations in pharmaceutical R&D and manufacturing as well as discuss future opportunities for improvements in the field.

This will be followed by a talk at 3:30 pm by Christopher Welch at the Indiana Consortium for Analytical Science and Engineering (ICASE) on the early development in the chromatographic separation of isomers. He will focus on Professor William H. Pirkle’s (1934–2018) impact on modern chemistry by inventing and popularizing widely used techniques for the analysis and purification of enantiomers—contributions that paved the way for the subsequent advances in the discovery, development, and manufacture of enantiopure pharmaceuticals, agrochemicals, and specialty chemicals.

The session will wrap up with a talk at 4:05 pm by Mengling Wong of Genentech on advancing chiral analysis and separation technology to support research drug discovery programs. Genentech’s Analytical Research group has developed an optimized chiral screening workflow that utilizes a set of 15 chiral phases. This chiral screen uses short columns, short run times, and high flow rates to achieve rapid, high-quality results. Wong will explain how this approach enabled Genentech to rapidly screen and then scale-up to purify milligram to multigram quantities of chiral molecules in support of their drug discovery programs.

Link to the full session: