Bioassay techniques are pivotal in quantifying and characterizing the effects of substances on biological systems. Utilized extensively in biotherapeutics, diagnostics, and environmental sciences, these assays range from ELISA for protein and antibody detection to flow cytometry for cell health analysis. Bio-layer interferometry and cell-based assays further elucidate drug-receptor interactions.
Ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) tracks blood metabolite changes after repetitive transcranial magnetic stimulation (rTMS)-cognitive training therapy.
Revisiting insertion distance for methanol and methylene chloride in GC analysis and confirming that insertion depth strongly affects response and reproducibility.
A novel SFC–QTOF-MS method accurately detected 10 drugs in oral fluid, matching LC–MS/MS in 83 DUID cases while reducing run time by 42% and organic solvent use by 81%.
Supercritical fluid extraction (SFE) and high-pressure liquid chromatography (HPLC) team up to recover olive waste antioxidants. Marina Russo of the University of Messina (Italy) discusses the details.
Waters' Erin Chambers says combining column chemistry and particle-physics innovation cut a GLP-1 impurity profiling workflow from three hours to under one.