A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography.
A research group at Lund University (Lund, Sweden) fused a multifunctional peptide tag to the N-terminal ends of green fluorescent protein, lactate dehydrogenase, and human hemoglobin in preparation for separation via ion-exchange chromatography, immobilized metal-ion affinity chromatography, and hydrophobic interaction chromatography. The peptide tag consisted of histidine, tyrosine, and aspartate residues. They found the tagged green fluorescent protein to be retained longer in both hydrophobic interaction and ion-exchange chromatography.
Dispersive Liquid-Liquid Microextraction: A Review and Roundup of Green Sample Prep Advancements
May 15th 2024The still relatively new technique has distinct advantages, but a few of those benefits make it incompatible with some of the currently accepted principles of green sample preparation.
Reflecting on the Influence of the Current State of Sample Preparation on GC, Part 2: Techniques
May 9th 2024In this installment, we examine trends in the use of sample preparation techniques through the lens of instrumental analysis by gas chromatography (GC) and GC–mass spectrometry (GC–MS).
Dispersive Liquid-Liquid Microextraction: A Review and Roundup of Green Sample Prep Advancements
May 15th 2024The still relatively new technique has distinct advantages, but a few of those benefits make it incompatible with some of the currently accepted principles of green sample preparation.
Reflecting on the Influence of the Current State of Sample Preparation on GC, Part 2: Techniques
May 9th 2024In this installment, we examine trends in the use of sample preparation techniques through the lens of instrumental analysis by gas chromatography (GC) and GC–mass spectrometry (GC–MS).