Reseachers Detect Bladder Cancer in Human Urine with HPLC/MS
Researchers conducting clinical research trials of patients with bladder cancer have discovered a non-invasive way to detect the cancer by using HPLC/MS.
Researchers conducting clinical research trials of patients with bladder cancer have discovered a non-invasive way to detect the cancer by using HPLC/MS. In the current study using sophisticated statistical methods the researchers were able to identify and characterize urine metabolite profiles obtained from patients with bladder cancer over controls. Identification and quantitative measurements of all the metabolites expressed in urine, serum, plasma, and tissue are essential for the study of biological processes in normal and disease states. High performance liquid chromatography was used to resolve the metabolites in urine that were detected by mass spectrometry according to their mass-to-charge ratio. Mass spectrometry was selected for this study because of its sensitivity.
The next step in their research will be the identification of key metabolites that are typical to patients with superficial bladder cancer. This may help to improve and simplify diagnosis of patients with bladder tumors. Furtherer more it will help to better understand their role in the pathophysiology of the disease and possible as a future target for therapeutic intervention.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
