Scientists from Taiwan Develop Compatible Method for DLLME with Reversed-Phase HPLC
Scientists from Taiwan have developed a method for dispersive liquid-liquid microextraction of ionisable compounds that renders it compatible with reversed-phase HPLC by combining it with in-syringe back extraction. The new system was demonstrated with the removal of the drug clenbuterol from water.
Scientists from Taiwan have developed a method for dispersive liquid-liquid microextraction (DLLME) of ionisable compounds that renders it compatible with reversed-phase HPLC by combining it with in-syringe back extraction. Ming-Ren Fuh and Mahaveer Melwanki from Soochow University in Taipei introduced in-syringe extraction as the final step to produce an analyte solution suitable for HPLC. They illustrated the technique with the extraction of clenbuterol from water. Fuh and Melwanki regard the speed of this combined extraction process to be the major advantage, the longest step being centrifuging the sample to collect the droplets of dispersed solvent.
Troubleshooting Everywhere! An Assortment of Topics from Pittcon 2025
April 5th 2025In this installment of “LC Troubleshooting,” Dwight Stoll touches on highlights from Pittcon 2025 talks, as well as troubleshooting advice distilled from a lifetime of work in separation science by LCGC Award winner Christopher Pohl.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
