Separation of Five Steroids Hamilton PRP -C18 Reversed-Phase HPLC Column

June 1, 2017
Hamilton Company

The Application Notebook

Volume 35, Issue 6



Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.

Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a reversed phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

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Hamilton Company
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Website: www.hamiltoncompany.com