|Articles|June 1, 2017
- The Application Notebook-06-01-2017
- Volume 35
- Issue 6
Separation of Five Steroids Hamilton PRP -C18 Reversed-Phase HPLC Column
Author(s)Hamilton Company
Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.
Steroids share a characteristic, polycyclic structure and have varying degrees of lipophilicity (log P). In this study, a reversed phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.
For more information on Hamilton HPLC columns and accessories or to order a product, please visit
Hamilton Company
4970 Energy Way, Reno, NV 89502
tel. (775) 858-3000, (800) 648-5950
Website:
Articles in this issue
over 8 years ago
Online MALS-QELS (Quasi-Elastic Light Scattering)over 8 years ago
Low Molecular Weight Polystyrene Characterized by SEC-MALSover 8 years ago
Analysis of Amino Acids in Oxidized and Unoxidized Feed Samplesover 8 years ago
GC×GC–FID for Routine Analysis of Total Petroleum HydrocarbonsNewsletter
Join the global community of analytical scientists who trust LCGC for insights on the latest techniques, trends, and expert solutions in chromatography.
Trending on LCGC International
1
Navigating Adverse Reactions in GLP-1 Treatment
2
Decoding Sweet Potato Drought Resilience: Metabolomic Evidence from UPLC-MS Profiling
3
Toward Safer Diabetes Devices: GC–MS Detection of Allergens in Sensors, Pumps, and Infusion Sets
4
Shifting GLP-1 Receptor Agonists from Proteins to Small Molecules
5
