Separation of Five Steroids on a Hamilton PRP-C18 Reversed-Phase HPLC Column

February 1, 2019
Hamilton Company
LCGC North America
Volume 37, Issue 2
Page Number: 141

In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.

Steroids represent a chemically distinct class of hormones with wide-ranging biological functions. Synthetic derivatives of endogenous steroid prototypes are used medically in birth control and in the treatment of asthma, arthritis, inflammation, and osteoporosis.


Figure 1: Five steroids on Hamilton PRP-C18

Steroids share a characteristic polycyclic structure, and have varying degrees of lipophilicity (log P). In this study, a reversed-phase HPLC method was developed for separation of five steroid hormones with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone) on a Hamilton PRP-C18 HPLC column.


Figure 1: Five steroids on Hamilton PRP-C18

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