Commission Regulation (EC) No 152-2009, published in TheOfficial Journal of European Union, laid down the methods of sampling and analysis for the official control of feed. The regulation describes methods of analysis to control the composition of feed materials and compound feed products. Establishing the amino acids profile is an important way to control quality and nutritional value of feeds. This regulation specifies HPLC with post-column derivatization with ninhydrin reagent as the method of analysis for total and free amino acids. Pickering Laboratories developed an analytical method to comply with all the chromatographic requirements of Commission Regulation (EC) No 152-2009. The same method is used to analyze oxidized and unoxidized feed samples.
Figure 1: Chromatogram of a standard solution of amino acids
Figure 2: Chromatogram of an oxidized feed sample
Figure 3: Chromatogram of a non-oxidized feed sample
Table I: HPLC Program
Table 2: Column oven program
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