Seven Must Know Points to Understand Atmospheric Pressure Chemical Ionization
CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.
CHROMacademy delivers key knowledge quickly in bite-size modules, and to prove this here is our seven point guide to understanding atmospheric pressure chemical ionization.
1) Atmospheric pressure chemical ionization (APCI) uses gas-phase ion-molecule reactions at atmospheric pressure. It relies on the formation of a plasma of ions comprised, mainly, from the HPLC mobile phase components.
2) In APCI the eluent is introduced into the interface using a capillary of similar design to the ESI source. However, no potential is applied to the capillary, instead the liquid emerges from the capillary surrounded by a flow of inert nebulizing gas into a heated, vaporizing region.
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Reversed Phase HPLC for the Analysis of Biomolecules
November 15th 2016Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served, at best, by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in the analysis of biomolecules. A subsequent article will detail the application of HILIC, IEX, and SEC chromatography for the analysis if biomolecules.
