Application Notes: Biopharmaceuticals

This application note describes the improved identification of pharmaceutical low-level impurities when using SFC and UHPLC as orthogonal separation modes.

From siRNA to mRNA to DNA, separating full length product from impurities can be challenging. Taking into consideration the nature of the oligonucleotide you are interested in, you will be able to make informed choices about what approach to use to characterize or purify your molecule.

This application note describes the company’s range of oligonucleotide columns and oligonucleotide standards.

This application note demonstrates the suitability of the company’s stationary phases to separate deprotected RNA and DNA oligonucleotides of different sizes.

A method using the company’s system to purify long-and short-term oligonucleotides is described.

This application note demonstrates direct ion-pair reversed-phase online desalting using 2D -LC for the analysis of oligonucleotides.

Polymeric strong anion exchange methods such as PL SAX are suitable for anion exchange and purification of oligonucleotides. At elevated temperatures, enhanced mass transfer increases the resolution for larger oligonucleotides. Working at higher PH values enables resolution of phosphodiester and phosphothioate forms of the oligonucleotide.

This application describes the purification of paracetamol from a crude sample in early-stage drug discovery. Outstanding purity (>99%) and recovery (>95%) are achieved.

This application note describes the improved identification of pharmaceutical low-level impurities when using SFC and UHPLC as orthogonal separation modes.

Dans cette note d’application vous découvrirez le QTof 6545XT et le logiciel BioConfirm 10.0 pour l’identification et la quantification des modifications post traductionnelles.

Cette brochure explique comment Agilent prend en charge l'ensemble du flux de travail des glycans de la préparation des échantillons à l'analyse LC, FLD, MS, et CE.

Téléchargez cette application pour connaître notre solution à la caractérisation de protéine native complexe non covalent par HPLC SEC–MS : Application aux mAb, ADC, et complexes protéiques.

Ce compendium couvre les techniques analytiques utilisées dans l’identification et la quantification des attributs de qualité critiques sur l’exemple de l’anticorps monoclonal NIST.

This application demonstrates the efficacy of this chromatographic technique for a fast orthogonal analysis to evaluate mAb ADCC activity.

Learn more about our HPLC-QTOF solution for the identification and quantification of post-translational changes for the development of therapeutic antibodies.

In this applications resource you'll know how Agilent supports your entire released glycan workflow from sample preparation to LC-FLD–MS and CE analysis.

Download this educational resource which covers applications for characterizing critical quality attributes

Discover our latest application note on native protein analysis by steric exclusion chromatography and high-resolution mass spectrometry.

Our compilation of Avantor® ACE® applications for key analyte classes in cannabis products and potential contaminants provides technical support for this key expanding area.

The TSKgel UP-SW Aggregate 30 nm column demonstrates the advantages of using a larger pore size when analyzing high-order aggregates and large molecules.

Doxorubicin is a well-established chemotherapeutic agent frequently used in cancer treatment. Here we present the application of Electrical Asymmetrical Flow Field-Flow Fractionation (AF4) coupled with Multi Angle Light Scattering (MALS) to derive essential physico-chemical parameters, such as size distribution and surface charge, of a commercial liposomal Doxorubicin formulation.

Doxorubicin is a well-established chemotherapeutic agent frequently used in cancer treatment. Here we present the application of Electrical Asymmetrical Flow Field-Flow Fractionation (AF4) coupled with Multi Angle Light Scattering (MALS) to derive essential physico-chemical parameters, such as size distribution and surface charge, of a commercial liposomal Doxorubicin formulation.

In this study we demonstrate the benefits of the Thermo Scientific™ Orbitrap Exploris™ 240 LC–MS/MS for intact biopharmaceutical analysis under native conditions.

PharmaFluidics’ μPAC capLC is evaluated for different user needs ranging from 12 samples per day in comprehensive mode to 65 samples per day in throughput mode.

PharmaFluidics introduces a new μPAC capLC column that offers unprecedented performance and that given its low backpressure can be used with flow rates of up to 15μL/min.

Monitoring the excellent separation performance of PharmaFluidics’ µPAC columns by using peptide retention time calibration mixtures for bottom-up proteomics.