Application Notes: Biopharmaceuticals

This application note presents the characterization of ribosomal RNA using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering.

Electrical asymmetrical flow field-flow fractionation coupled with MALS was used to characterize the NIST monoclonal antibody reference material 8671.

This application note presents the characterization of ribosomal RNA using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering.

This study describes the characterization of metal-containing nanoparticles in tattoo ink using AF4 coupled with multi-angle light scattering and ICP-MS.

This study describes the characterization of metal-containing nanoparticles in tattoo ink using AF4 coupled with multi-angle light scattering and ICP-MS.

⦁ How to use the ACQUITY APC system with aggressive solvent compatible p-QSM technology to support gradient polymer elution chromatography (GPEC). ⦁ Selecting the appropriate detector for enhanced performance and insight.

⦁ How equipping LC with multi-detection technology enables achieving high quality, high-resolution, and rapid sample analysis. ⦁ Leverage LS and RI detectors and viscometer technologies to calculate absolute molecular weight, hydrodynamic radius, and structure of the materials.

⦁ Determine polymer properties such as molecular weight, dispersity, and branching/conformation. ⦁ How to differentiate molecular structure across the molecular weight distribution.

⦁ Reduce run time and solvent consumption by scaling both isocratic and gradient methods using the Alliance HPLC. ⦁ Utilization of the Waters Columns Calculator for scaling isocratic and gradient methods.

⦁ Benefits of utilizing a custom library in the targeted analysis of migration samples. ⦁ Insight into how proposed workflow is used in identifying targeted and untargeted photoinitiators in migration studies.

With Antec Scientific’s new µ-PrepCell-SS, electrochemical reduction of disulfide bonds in proteins, mAbs, and other biopharmaceuticals is possible in any LC-MS workflow.

This note demonstrates the ability of a TSKgel® UP-SW2000 SEC column to discriminate oligonucleotides differing by one base in length.

With Antec Scientific’s new µ-PrepCell-SS, electrochemical reduction of disulfide bonds in proteins, mAbs, and other biopharmaceuticals is possible in any LC-MS workflow.

Statins were analyzed without using reversed phase mode separation nor ion-pairing reagents.

Shodex RSpak DE-413L is suitable for the analysis of water-soluble vitamins in a single run.

µPAC™ technology has been used for analysing HCP impurities originating from recombinant expression systems used in the production of mAb based biotherapeutics.

All data is a cGMP record, as regulators enforce requirements to ensure the integrity of laboratory data. Find out how LabVantage Solutions can aid in compliance.

• Improving sample preparation improves sensitivity and reliability • Using a high-capacity paramagnetic bead solution to clean up & isolate mAbs • Optimize clean-up of labeling reagent, salts, or surfactants pre-analysis

μPAC™ technology has been used for analysing HCP impurities originating from recombinant expression systems used in the production of mAb based biotherapeutics.

The landscape for new biological drug development continues to evolve in complexity. With biosimilars entering the market and originator products coming off patent, the competitive race for market dominance is reliant on!

The landscape for new biological drug development continues to evolve in complexity. With biosimilars entering the market and originator products coming off patent, the competitive race for market dominance is reliant on!

This note describes how SEC-MALS-UV-RI analysis quantifies multiple critical quality attributes of AAV viral vectors, including concentration/titer, aggregation and empty:full ratio.

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.

The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.

This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.

In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.

This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.