Application Notes: Biopharmaceuticals

The dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the MAb monomer from its aggregates as well as from degradation products in a single run.

The less hydrophobic wide-pore column YMC-Triart Bio C4 is used for the analysis of modified oligonucleotides due to the hydrophobic character of the disulfide units.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial MAbs by micro-LC- MS/MS at an elevated temperature of 75°C.

This application note shows how to achieve narrow peaks and high sensitivity by optimising the AEX separation of phosphorothioate oligonucleotides (PS) in only 5 steps.

In this application note eight intact proteins in the protein mix MSRT2 are analysed by Micro-LC–MS using an YMC-Triart Bio C4 capillary column.

This application note using YMC-Triart Bio C4 shows a robust separation at high temperatures of 3 commercially available MAbs: Ofatumumab, Palivizumab, and Panitumumab.

In this application YMC-Triart Bio C4 was used to analyse a reduced monoclonal antibody and a reduced ADC mimic with formic acid or TFA as additives.

This application demonstrates the efficacy of this chromatographic technique for a fast orthogonal analysis to evaluate mAb ADCC activity.

When SEC does not provide optimal separation of proteins for characterization by multi-angle light scattering, MALS can be coupled to ion-exchange chromatography.

This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.

The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.

A simple and sensitive method is presented for the direct analysis of Glyphosate and AMPA in drinking water without sample derivatization or pre-concentration.

This white paper discusses the advantages of using fast, efficient HALO® Fused-Core® Technology columns and manipulating selectivity to quickly develop methods, for fast high efficiency LC and LCMS separations.

• Special considerations when updating software • Data integrity challenges in the cloud environment • Implications of moving to the cloud with respect to audits

• Validating custom calculations • The importance of a second-person review and locking data • How to compile a data-integrity plan · · ·

-How to translate methods, maximize resolution, and comply with USP -Optimizing HPLC/UHPLC method development workflows -Column coupling can provide improved sample detail and high peak capacities

Multidimensional LC/MS is a powerful tool to gain info in the laboratory. Analyzing ADCs with multidimensional LC/MS provides fast data for CQAs.

- ADCs can be analyzed using various methods other than the traditional HIC. - New chromatographic materials expand analytical techniques for APOC

The applicability of AF4-UV-MALS for sizing and quantification of adeno-associated virus (AAV) samples with different degrees of aggregation is presented.

This study investigates the capabilities of asymmetrical flow field-flow fractionation coupled with MALS for the detection of carbon nanotubes in soot and soil.

The applicability of AF4-UV-MALS for sizing and quantification of adeno-associated virus (AAV) samples with different degrees of aggregation is presented.

This study investigates the capabilities of asymmetrical flow field-flow fractionation coupled with MALS for the detection of carbon nanotubes in soot and soil.

This study demonstrates the combination of inverse supercritical fluid extraction and miniaturized AF4-MALS for the analysis of nanoparticles in commercial sunscreens.

This study demonstrates the combination of inverse supercritical fluid extraction and miniaturized AF4-MALS for the analysis of nanoparticles in commercial sunscreens.

This study describes the analysis of PLGA particles under physiological conditions using centrifugal field-flow fractionation coupled with dynamic light scattering.

This study describes the analysis of PLGA particles under physiological conditions using centrifugal field-flow fractionation coupled with dynamic light scattering.