Application Notes: Biopharmaceuticals

YMC’s latest HIC column is used to analyse the DAR of Brentuximab vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

The dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the MAb monomer from its aggregates as well as from degradation products in a single run.

The less hydrophobic wide-pore column YMC-Triart Bio C4 is used for the analysis of modified oligonucleotides due to the hydrophobic character of the disulfide units.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial MAbs by micro-LC- MS/MS at an elevated temperature of 75°C.

This application note shows how to achieve narrow peaks and high sensitivity by optimising the AEX separation of phosphorothioate oligonucleotides (PS) in only 5 steps.

In this application note eight intact proteins in the protein mix MSRT2 are analysed by Micro-LC–MS using an YMC-Triart Bio C4 capillary column.

This application note using YMC-Triart Bio C4 shows a robust separation at high temperatures of 3 commercially available MAbs: Ofatumumab, Palivizumab, and Panitumumab.

In this application YMC-Triart Bio C4 was used to analyse a reduced monoclonal antibody and a reduced ADC mimic with formic acid or TFA as additives.

This application demonstrates the efficacy of this chromatographic technique for a fast orthogonal analysis to evaluate mAb ADCC activity.

When SEC does not provide optimal separation of proteins for characterization by multi-angle light scattering, MALS can be coupled to ion-exchange chromatography.

This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.

The molecular similarity between Humira® and Yervoy® biosimilars and the corresponding innovator reference products is examined using analytical SEC, RPC and HIC.

A simple and sensitive method is presented for the direct analysis of Glyphosate and AMPA in drinking water without sample derivatization or pre-concentration.

This white paper discusses the advantages of using fast, efficient HALO® Fused-Core® Technology columns and manipulating selectivity to quickly develop methods, for fast high efficiency LC and LCMS separations.

• Special considerations when updating software • Data integrity challenges in the cloud environment • Implications of moving to the cloud with respect to audits

• Validating custom calculations • The importance of a second-person review and locking data • How to compile a data-integrity plan · · ·

-How to translate methods, maximize resolution, and comply with USP -Optimizing HPLC/UHPLC method development workflows -Column coupling can provide improved sample detail and high peak capacities

Multidimensional LC/MS is a powerful tool to gain info in the laboratory. Analyzing ADCs with multidimensional LC/MS provides fast data for CQAs.

- ADCs can be analyzed using various methods other than the traditional HIC. - New chromatographic materials expand analytical techniques for APOC

The applicability of AF4-UV-MALS for sizing and quantification of adeno-associated virus (AAV) samples with different degrees of aggregation is presented.

This study investigates the capabilities of asymmetrical flow field-flow fractionation coupled with MALS for the detection of carbon nanotubes in soot and soil.

The applicability of AF4-UV-MALS for sizing and quantification of adeno-associated virus (AAV) samples with different degrees of aggregation is presented.

This study investigates the capabilities of asymmetrical flow field-flow fractionation coupled with MALS for the detection of carbon nanotubes in soot and soil.

This study demonstrates the combination of inverse supercritical fluid extraction and miniaturized AF4-MALS for the analysis of nanoparticles in commercial sunscreens.

This study demonstrates the combination of inverse supercritical fluid extraction and miniaturized AF4-MALS for the analysis of nanoparticles in commercial sunscreens.

This study describes the analysis of PLGA particles under physiological conditions using centrifugal field-flow fractionation coupled with dynamic light scattering.

This study describes the analysis of PLGA particles under physiological conditions using centrifugal field-flow fractionation coupled with dynamic light scattering.