Application Notes: Biopharmaceuticals

The method development for the simultaneous determination of highly hydrophobic Amyloid β peptides is described.

SEC-MALS analyzes proteins, peptides, nucleic acids, polysaccharides and bionanoparticles to determine molar mass, size, conjugation, aggregation and interactions.

• Challenges and solutions for getting the most accurate data from SEC • Combining SEC with UV or MS detection to quickly analyze samples

The benefits of using QuanRecovery Vials for trace analysis of fatty acids in RPLC-MS based workflows.

In this application note, infliximab and trastuzumab were separated on a BioResolve SCX mAb Column, and their charge variant fractions were collected using a Waters Fraction Manager-Analytical (WFM-A).

This document is designed to help the user performing intact and/or subunit protein analysis evaluate the performance or suitability of the instrument by undertaking a simple system check (SC) and running of QC standards.

The benefits of using QuanRecovery Vials for trace analysis of fatty acids in RPLC-MS based workflows.

In this application note, infliximab and trastuzumab were separated on a BioResolve SCX mAb Column, and their charge variant fractions were collected using a Waters Fraction Manager-Analytical (WFM-A).

This document is designed to help the user performing intact and/or subunit protein analysis evaluate the performance or suitability of the instrument by undertaking a simple system check (SC) and running of QC standards.

• Intact workflow solutions for characterizing biomolecules by HIC and SEC • AdvanceBio HIC columns offer new levels of hydrophobicity and selectivity

• 4D LC offers significant benefits for biotherapeutic characterization. • Multidimensional LC can help find previously undetected impurities. • User-friendly software offers a solution to 4D-LC challenges.

In this webinar, learn how to achieve cleaner, more concentrated, and targeted sample preparation methods to help with downstream analysis for both analytical and biopharmaceutical labs.

In this webinar, learn how to achieve cleaner, more concentrated, and targeted sample preparation methods to help with downstream analysis for both analytical and biopharmaceutical labs.

• Applications of native mass spectrometry • Implementation and technical challenges with native MS

In this application note we look at the impact that mobile phase concentration has on the sensitivity of SEC-MS when used to analyze intact mAb’s under non-denaturing conditions.

In this application note we look at the impact that mobile phase concentration has on the sensitivity of SEC-MS when used to analyze intact mAb’s under non-denaturing conditions.

In this application note we show the separation of charge variants of Trastuzumab under native conditions using a pH gradient formed with volatile buffers.

In this application note we look at a method for determining the level of aggregation in a commercially available sample of intact NIST mAb using a bioZen SEC-2 column together with the SCIEX X500B mass spec.

This application note serves to highlight how native MS can be used to get intact mass for non-covalently linked large molecules, including cysteine linked ADCs

In this application note we show the separation of charge variants of Trastuzumab under native conditions using a pH gradient formed with volatile buffers.

In this application note we look at a method for determining the level of aggregation in a commercially available sample of intact NIST mAb using a bioZen SEC-2 column together with the SCIEX X500B mass spec.

In this application we show the separation of several charge variants of the NIST mAb under native ion exchange and mass spectrometry (high resolution MS) conditions using a pH gradient formed from volatile buffers.

In this application note we show high resolution separation for a suite of protein standards under native mass spec conditions typical for SEC.

In this application note we show high resolution separation for a suite of protein standards under native mass spec conditions typical for SEC.

This application note serves to highlight how native MS can be used to get intact mass for non-covalently linked large molecules, including cysteine linked ADCs

Combining the efficient immunocapture and sample extraction of bioZen MagBeads, trastuzumab is isolated from rat plasma to yields greater selectivity relative to traditional ligand-binding assays.

Combining the efficient immunocapture and sample extraction of bioZen MagBeads, trastuzumab is isolated from rat plasma to yields greater selectivity relative to traditional ligand-binding assays.

In this application, Streptavidin coated bioZen MagBeads are utilized for the fast and accurate immunocapture of insulin aspart novolog. By combining immunocapture sample preparation with LC MS/MS, this method demonstrates a functionalized assay that yields greater selectivity and sensitivity.