UltraPerformance LC (UPLC) has been widely accepted by chromatographers because of improvements over HPLC in the sensitivity, resolution and speed of separations. As scientists begin to use this technology for impurity and metabolite profiling, they will need to transfer the methods to preparative LC to isolate and purify their compounds for further research. Therefore, it is necessary to systematically transfer UPLC assays not only to HPLC, but, more importantly, to preparative chromatography. In this application, we provide information on how to scale a UPLC impurity/degradant separation to a preparative LC separation.
Eric S. Grumbach, Diane M. Diehl and Jeffrey R. Mazzeo, Waters Corporation
The use of a silica column for HILIC is examined, including mechanistic studies of analyte retention on silica in aqueous-organic mobile phases.
This article describes a method development approach that uses different packing materials and mobile-phase pH levels to optimize the chromatographic separation of degradation products from active ingredients.