Jeffrey R. Mazzeo | Authors


Seamless Method Transfer from UPLC Technology to Preparative LC

UltraPerformance LC (UPLC) has been widely accepted by chromatographers because of improvements over HPLC in the sensitivity, resolution and speed of separations. As scientists begin to use this technology for impurity and metabolite profiling, they will need to transfer the methods to preparative LC to isolate and purify their compounds for further research. Therefore, it is necessary to systematically transfer UPLC assays not only to HPLC, but, more importantly, to preparative chromatography. In this application, we provide information on how to scale a UPLC impurity/degradant separation to a preparative LC separation.

Hydrophilic Interaction Chromatography Using Silica Columns for the Retention of Polar Analytes and Enhanced ESI-MS Sensitivity

The use of a silica column for HILIC is examined, including mechanistic studies of analyte retention on silica in aqueous-organic mobile phases.

A Four-Column Approach to Developing Stability-Indicating HPLC Methods

This article describes a method development approach that uses different packing materials and mobile-phase pH levels to optimize the chromatographic separation of degradation products from active ingredients.