Jason A. Anspach

In this study, we compare the performance of plastic and metal materials in UHPLC columns designed for the analysis of biological molecules. We evaluate the performance of these materials in terms of inertness, column chromatographic performance, and reproducibility.

Ultrahigh-pressure liquid chromatography (UHPLC) columns are used in peptide mapping to improve the resolution of highly complex peptide mixtures. It is commonly assumed that small particle columns increase performance, but this is not always the case. This study presents a comparison of peak count, column length, and resolution between core–shell and fully porous UHPLC columns.