In this study, we compare the performance of plastic and metal materials in UHPLC columns designed for the analysis of biological molecules. We evaluate the performance of these materials in terms of inertness, column chromatographic performance, and reproducibility.
This article describes the analysis of fluorescently labelled N-glycans released from proteins by liquid chromatography–mass spectrometry (LC–MS).
The new 1 mm i.d. ProSwift strong cation-exchange (SCX) column is a polymethacrylate-based monolithic column with sulfonate functionality.
Dionex Application Note
The new reversed-phase ProSwiftÂ® 1 mm i.d. column is a divinylbenzene-based monolithic column for routine chromatography of proteins and other biomolecules. It is available in two different lengths. The shorter (1 Ã- 50 mm) format is designed for fast separations and the longer (1 Ã- 250 mm) format is intended for high resolution analytical separations. However, depending on the application, either can be used for separation of proteins and for coupling with mass spectrometry.