Pilar Franco

Therapeutic peptides represent one of the fastest growing segments in the pharmaceutical market. To bring these products to the market in a consistent manner, high quality is a major concern and requires stringent quality control (QC) methods. This article discusses the potential of zwitterionic chiral ion-exchangers to support peptide analysis and quality control as a flexible complementary tool to monitor the stereochemical integrity and chemical modifications.

Quinine- and quinidine-derived anion-exchanger chiral stationary phases have proven to be versatile in enantiomer separation of acidic compounds in HPLC. In this article, the authors demonstrate their performance in specific HPLC applications involving enantiomer resolution and topoisomer separation.

The development and application of chiral chromatography is described.

Chiral chromatography is now the fastest way to access large amounts of enantiopure pharmaceutical compounds.

The authors describe a study of screening procedures that demonstrated a relatively small set of columns can be used routinely.

A relatively small set of columns can be used on a routine basis in different modes to meet modern analytical needs.

To meet the growing need for fast reversed-phase enantiomer separations, two new 3-μm reversed-phase columns, CHIRALCEL® OD® -3R and CHIRALPAK® AD® -3R, have been introduced. High column performance and column stability under a wide range of conditions, including aqueous solvent systems suited to LC–MS, have been

The move from conventional particle sizes (5 μm or higher) to smaller diameter packing materials is one of the most attractive approaches to achieve higher separating efficiency. Recently developed 3 μm polysaccharide-derived chiral stationary phases demonstrate characteristics of favourable mass transfer kinetics, high column efficiency and good column permeability. This allows the fast analysis of enantiomers using conventional HPLC instruments.