Assessing Protein Aggregation with SEC-MALS
Determine the molecular weight of low abundant species with confident with the highly sensitive DAWN MALS detector and improved peak separation with Waters BEH Column.
Analysis of mAbs, biosimilars and AAVs
Explore SEC-MALS-RI multi-attribute quantification of antibodies, biosimilars, and AAVs.
SEC-MALS and Fluorescence detection of AAV
Explore the optimization of AAV separation and the combined intrinsic fluorescence detection with SEC-MALS for low-concentration measurement.
Optimizing Development and Release Efficiency of Protein, AAV, and LNP Therapies Using Multi-Attribute Quantification (MAQ) Light Scattering
Webinar Date/Time: Wed, Nov 1, 2023 11:00 AM EDT
SEC-MALS Method for Characterizing mRNA (Dec 2022)
SEC-MALS quantifies biophysical attributes of mRNA, including molar mass, percent aggregates, Rg, and Rh, and how they are affected by formulation and storage.
Characterizing Lipid Nanoparticles (LNPs) for RNA Delivery
Field-flow fractionation coupled to multi-angle light scattering (FFF-MALS) is a powerful analytical tool that determines multiple CQAs of LNP-RNA nanoparticles.
Characterizing Branched Polymers by Light Scattering
SEC with multi-angle light scattering (SEC-MALS) or in conjunction with a viscometer (SEC-MALS-IV) effectively characterizes branching in polymers.
SEC–MALS Method for Characterizing mRNA (Sept 2022)
SEC–MALS quantifies biophysical attributes of mRNA, including molar mass, percent aggregates, Rg, and Rh, and how they are affected by formulation and storage conditions.
Characterizing AAVs with Light Scattering
Wyatt’s instruments and techniques constitute platform methods for effective product and process development, as well as quality control of AAVs for gene therapy.
Light Scattering Toolkit for Vaccines
Light scattering facilitates vaccine development by enabling the analysis of size, shape, molar mass, and other parameters derived from these properties.
Characterizing AAVs with SEC-MALS
SEC-MALS is a platform method for effective product and process development, as well as quality control, of AAVs for gene therapy.
Quantifying Protein PEGylation Processes
SEC–MALS characterizes PEGylated proteins to determine the protein-PEG ratio and the molar masses of the constituent protein and PEG at each eluting fraction.
Understanding Antibody and Viral Glycoprotein Interactions (Sept 2022)
SEC-MALS and CG-MALS characterize viral glycoprotein (vGP) self-association and the absolute stoichiometry and affinity of vGP: antibody interactions.
Characterizing Lentiviral and Gammaretroviral Vectors
This note demonstrates the use of DLS and online multi-angle light scattering with field-flow fractionation (FFF-MALS) for characterizing retroviral viral vectors.
Lipid Nanoparticle and Liposome Quantification
Field-flow fractionation with multi-angle and dynamic light scattering (FFF–MALS–DLS) combines size-based separation with absolute size and structure determination.
LNP Characterization in Product Development
Light scattering quantifies key attributes of lipid nanoparticle gene vectors such as payload, size, titer, and stability in formulation and physiological media.
Advances in Downstream PAT for Biologics, Vaccines, and Gene Vectors
Tue, Aug 23, 2022 11:00 AM EDT Advanced PAT tools such as RT–MALS offer in-depth characterization of biological molecules and nanoparticles to help optimize manufacturing processes.
Characterizing Vaccines with Light Scattering (August 2022)
Light scattering methods inform on essential properties and attributes of vaccines including size, aggregation, stability, interactions, composition, and conformation.
Characterization of Retroviral Vectors
Large viral vectors are characterized rapidly using dynamic light scattering and in-depth by multi-angle light scattering in combination with field-flow fractionation.
Characterization of Adeno-Associated Viral Vectors
Quality attributes of Adeno-Associated Virus (AAV) vectors are achieved with multi-angle light scattering coupled to separation methods and high-throughput dynamic light scattering.
SEC-MALS Method for Characterizing mRNA
SEC-MALS quantifies biophysical attributes of mRNA including molar mass, percent aggregates, Rg and Rh, and how they are affected by formulation and storage conditions.
SEC-MALS and CG-MALS: Complementary Techniques to Characterize Protein-DNA Complexes
This white paper presents the characterization of protein-DNA complexes using two complementary multi-angle light scattering techniques, SEC-MALS and CG-MALS.
Protein Characterization by IEX-MALS
IEX-MALS overcomes some of the restrictions of SEC-MALS that arise from SEC, while providing absolute molar mass, size, and quality assessment of heterogeneous proteins.
Characterizing Lentiviral and Gammaretroviral Vectors by Batch DLS and FFF-MALS
This note demonstrates the use of DLS and online multi-angle light scattering with field-flow fractionation (FFF-MALS) for characterizing retrovial viral vectors.
Identification of Insulin Oligomeric States
SEC-MALS identifies monomers, dimers, hexamers, and higher aggregates of insulin to determine optimal formulation, storage, and administration conditions.
AAV Application Notebook: Characterizing AAVs with Light Scattering
Wyatt’s instruments and techniques constitute platform methods for effective product and process development, as well as quality control, of AAVs for gene therapy.
Analysis of Linear and Branched Amylose
Branching analysis of amylopectin requires a linear amylose analog. Synthetic amylose was prepared and characterized by SEC-MALS to determine if branching is present.
Liposome Structure and Concentration by FFF-MALS-DLS
Field-flow fractionation with online static and dynamic light scattering constitutes a powerful and versatile system for nano-pharmaceutical characterization.
SEC-MALS Characterization of Block Copolymers
February 24th, 2022, at 8am PST | 11am EST | 4pm GMT | 5pm CET Join us for this informative webcast to learn how to illuminate block copolymers with SEC-MALS.
Conformational Properties of Hyperbranched Hetero-Polysaccharides v2
This note discusses the characterization of conformational properties of hyperbranched macromolecules by combining SEC-MALS with differential viscometry.