YMC America, Inc.

YMC America’s J Preston and Jeff Kakaley discuss the 5-in-1 functionality of YMC’s flagship chromatography system – the Contichrom® CUBE. They’ll explore using the instrument for different modes of chromatography, the added benefits of YMC resins, and how the CUBE can be used to scale up to YMC’s TWIN MCSGP systems.

YMC-Triart Bio C4 is used to successfully analyze monoclonal antibodies in reversed-phase mode in this robust application.

This application demonstrates that YMC-BioPro IEX SF is an outstanding stationary phase for the characterization of charge variants in mAbs.

This application note demonstrates the use of YMC-Triart C18 ExRS for UHPLC potency analysis of 11 common cannabinoid compounds.

Achieve narrow peaks and high sensitivity by optimizing the separation of phosphorothioate oligonucleotides in 5 steps using the strong anion exchanger BioPro IEX QF.

This application note explores when to use each of YMC’s two HIC columns by comparing retention behaviors of BioPro HIC HT and BioPro HIC BF.

This application note investigates the use of YMC BioPro SF for high-resolution charge variant analysis of four therapeutic mAb products.

The dial-a-mix method with a quaternary pump HPLC quickly screens different method conditions when developing ion-exchange methods for charge variant analysis of mAbs.

This application note describes the benefits of using YMC-Triart PEEK-lined columns for separations involving coordination compounds.

This application note investigates using YMC-Triart C18 for analysis of 11 common cannabinoid compounds.

This application note details real-life client data obtained for insulin purification and manufacturing using YMC’s new YMC-Triart Prep Bio200 C8 material.

This application note details the use of light scattering detection coupled with SEC analysis for characterization of proteins and antibodies.

Highlighting the use of the dial-a-mix method for screening different mobile-phase pH conditions during method development of IEX charge variant analysis.

This application note details the development of a HPLC method for analyzing CBD and Melatonin in an over-the-counter sleep aid.

This application note highlights the ability of YMC-Triart C18 to separate 11 common cannabinoids encountered during potency testing.

A loading study for purifying cannabidiol (CBD) from a commercially available “high-CBD” hemp oil extract

Demonstrating the use of YMC’s new 2µm Diol stationary phase for scaling down size exclusion analyses

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

Smaller particle size stationary phases have seen increased use in the modern analytical laboratory due to their ability to exhibit greater resolution while enabling the use of smaller column geometries. Shorter column lengths and narrower bores allow for faster run times and lower flow rates, decreasing laboratory costs in terms of solvent consumption and man-hours needed to run the analyses. This application note examines the effects of smaller particle sizes in various column geometries on size-exclusion chromatography (SEC) methods and the method variables affecting the characterization of antibody products.

The majority of the samples in this study and other studies show that a majority of compounds can be separated by the use of the three most common alcohols: IPA, CH3OH, and ethanol. Solubility becomes a crucial factor in preparative chromatography. Combinations of solvents can be used to improve solubility when the chosen co-solvent has poor solubility of the compound in question.

Silica-based cyano stationary phases have historically exhibited poor stability and short column lifetime at pH extremes. This application note details an investigation of the stability of YMC's Cyano-HG (high strength) 10 ?m silica-based stationary phase, which was performed at a customer's request.