Zeshan Aqeel | Authors

Gel filtration chromatography (GFC) is the most widely used method for quantitating protein aggregates in therapeutic drugs. It is a simple method, but prone to error as a result of poor method development and column selection. GFC columns tend to non-specifically adsorb large proteins and aggregates resulting in poor quantitation of “true” aggregate amount. Sample “priming” and mobile phase optimization can help reduce such irregularities. Simple method development rules using new column technologies are presented that demonstrate improved accuracy for these methods.

Phenomenex Application Note

This study evaluates the performance of a C18 core-shell phase that incorporates a C18 ligand with iso-butyl side chains. The Kinetex? XB-C18 HPLC/ UHPLC column delivers a fast and effective separation of several common preservatives in cosmetics.