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The Application Notebook-02-01-2010
The Application Notebook
Call for Application Notes
April 14, 2010
Article
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LCGC is planning to publish the next issue of The Application Notebook special supplement in June.
Improved Reproducibility of MW Measurements of Polypeptoids Using an EcoSEC GPC System
February 01, 2010
Polymer
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0
The EcoSEC GPC System and a set of TSK-GEL mixed-bed columns, both available from Tosoh Bioscience, were used successfully for obtaining high quality MWD data of a series of block poly-β-alkylalanoids with HFIP as the mobile phase in under 15 min.
Preparing for Pittcon 2010
February 01, 2010
From the Publisher
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There is an old axiom, attributed to various individuals, that holds: "Luck favors the prepared." This can apply to nearly any endeavor or project in life, as generally speaking, the harder you work, the more likely it is that good things will happen.
Stability of the RegisPack® Chiral Stationary Phase Under Reversed-Phase LC Conditions
February 01, 2010
Chiral
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The RegisPack® chiral stationary phase allows for the enantiomeric separations of a wide variety of racemate classes.
The Analysis of Melamine in Milk Powder Using HILIC with UV Detection
February 01, 2010
Food and Beverage
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The analysis of melamine in milk and other food products became urgent when first the pet food, and later the milk scandal were discovered in the U.S. and China.
Flexible Fused Silica Capillary: Impact of Aging on Strength
February 01, 2010
General
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One of the most important attributes of flexible fused silica capillary tubing is its outstanding strength. When made properly with a protective abrasion resistant coating and handled appropriately this material has proven to maintain its strength remarkably well.
Analysis of Basic Pharmaceuticals and Peptides on a Multi-Mode ODS Column
February 01, 2010
Pharmaceutical
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Analyzing basic pharmaceuticals and peptides can be challenging with conventional ODS columns.
U.S. EPA Method 524.3 for Analysis of Volatile Organic Compounds (VOCs) in Finished Drinking Water
February 01, 2010
Environmental
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The U.S. EPA Office of Groundwater and Drinking Water released Method 524.3, "Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry, Version 1.0," in the Federal Register on August 3rd, 2009 (1, 2).
Matrix Elimination- Inline Removal of Chloride Using InGuard Ag and Na Cartridges
February 01, 2010
General
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In ion chromatography, the presence of a large amount of matrix ions makes quantification of the target ions difficult.
Simplifying Peptide Bioanalysis
February 01, 2010
Biological
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Biomolecules, such as proteins and peptides, represent a growing number of pharmaceutical drug entities.
30-s UHPLC Separation of Pharmaceutical Ingredients Using PDA Detection
February 01, 2010
Pharmaceutical
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0
Ultra fast separations of acetaminophen, caffeine, propofol, and parabens are achieved in 30 s using the Accelaâ„¢ UHPLC system with the 80 Hz PDA detector.
Analysis of Semi-Volatile Organic Compounds (EPA Method 525) Using A Highly Versatile C18 Based Sorbent
February 01, 2010
Environmental
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0
The extraction and analysis of semi-volatile organic (SVOC's) compounds can be problematic due to the wide range of analyte types and polarities.
Analysis of N-Nitroso Glyphosate in Glyphosate Samples
February 01, 2010
Environmental
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Glyphosate (GP) is a non-selective, postemergence herbicide widely used for weed and vegetative control.
Quantitative Characterization of Polymer Blends by Pyrolysis-Gas Chromatography-Time of Flight Mass Spectrometry (Pyr-GC-TOFMS)
February 01, 2010
Polymer
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Pyrolysis GC?MS is a valuable tool for the material scientist. It is used principally for qualitative identification of polymers and their breakdown products. This application note displays how this technique can also be used for quantitative determination of polymer compositions by comparing peak area ratios of monomers and/or primary pyrolysis products.
Virus Particle Characterization
February 01, 2010
Biological
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0
Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.
The Qualitative Analysis of Evolved Gases in Thermogravimetry by Gas Chromatography Mass Spectrometry
February 01, 2010
Biological
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0
Thermogravimetric analysis (TGA) measures the change in the weight of a sample as a function of temperature.
17 Min D2712 Analysis of Impurities in Propylene Using PLOT Columns
February 01, 2010
Industrial
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Capillary PLOT columns can be a simpler alternative to packed column setups for propylene analysis. Here we demonstrate a single column setup that provides fast baseline resolution of propylene impurities.
Evaluation of Several Columns and Solvents for Gel Permeation Chromatography (GPC) Clean-Up of Fish Tissue Prior to PCB Analysis
February 01, 2010
Environmental
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The determination of PCBs, as well as other environmental contaminants, in fish tissue requires extensive sample cleanup prior to analysis by gas chromatography with an electron capture detector (GC-ECD).
Profiling of Essential Oil VOCs and SVOCs by GC–TMS
February 01, 2010
Industrial
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Spice and fragrance essential oil raw materials frankincense (Boswellia carterii) from India and Somalia, sandalwood (Santalum album) from India and Indonesia, and corn mint (Mentha haplocalyx) from the United States were analyzed for on-site analytical profiling and confirmation of quality and purity of therapeutic grade essential oils from raw materials.
Fractionation of Extractable Petroleum Hydrocarbons from Soil Using ISOLUTE® EPH SPE Columns
February 01, 2010
Environmental
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ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8–C40 aliphatics, C10–C22 aromatics).
High Resolution of Complex Lipids (Triglycerides) Using the Agilent 1290 Infinity LC and ZORBAX RRHT and RRHD 1.8 µm Columns
February 01, 2010
Food and Beverage
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The Agilent 1290 Infinity LC has significant capabilities for a wide range of HPLC and UHPLC applications.
Determination of Steviol Glycosides by HPLC with UV and ELS Detections Using the Acclaim Mixed-Mode WAX-1 Column
February 01, 2010
Food and Beverage
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The stevia plant and its extracts have long been used as sweeteners in Asia and Latin America. Two steviol glycosides present in plant tissue, stevioside, and rebaudioside A, are largely responsible for the sweet flavor (1).
An Empirical Evaluation of HILIC and Monolithic Columns for SFC Applications
February 01, 2010
General
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Separation of polar compounds by conventional reverse phase chromatography can be challenging due to their poor retention. Other HPLC approaches include normal phase chromatography (NPC) and its variation, hydrophilic interaction chromatography (HILIC).
High Resolution, Accurate Identification of Small Molecule Unknowns with UPLC and the maXis
February 01, 2010
Pharmaceutical
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Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques.
Increased Efficiency and Resolution with Kinetex„ Core-Shell Technology
February 01, 2010
General
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Over the past several years column manufacturers have been introducing columns packed with smaller particle sizes – sub-2 µm and 3 µm – to take advantage of the improvements that such small particle size columns offer.
EDTA by Anion Exchange HPLC
February 01, 2010
Pharmaceutical
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Hamilton Company is best known for manufacturing precision fluid measuring products, including syringes for HPLC and GC.
Generating Make-Up Gas for GC with an In-house Nitrogen Generator
February 01, 2010
General
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Since the gas flow required for the separation step in gas chromatography is frequently lower that that required to optimize the detection, nitrogen is used as a make-up gas to increase the gas flow for detection.
N-Methyl Carbamates in Water
February 01, 2010
Environmental
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N-methyl carbamates (NMCs) are widely used as pesticides and have been reported in the environment and food.
Ionic Liquids by IC–MS
February 01, 2010
General
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The characteristic composition of ionic liquids (an organic cation or anion and a counterion, in either organic or inorganic form) exhibits unique properties, such as extremely low vapor pressure, excellent thermal stability, electrical conductivity, high polarity, and miscibility with various types of solvents.
Analysis of Impurities in Fine Chemical Octyl-dimethyl-4- aminobenzoate Using the Agilent 1290 Infinity LC and ZORBAX RRHT and RRHD 1.8 µm Columns
February 01, 2010
General
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The combined benefits are demonstrated by a separation of impurities found in a sample of octyl-dimethyl-4-aminobenzoate.
Determination of Genotoxic Besylates and Tosylates by HPLC-ECD Using A Boron-Doped Diamond Electrode
February 01, 2010
Pharmaceutical
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Genotoxic impurities in pharmaceutical products have come under increasing regulatory scrutiny, with exposure limits
Requirements of an Automated Sample Delivery System in Today's Realm of Ever Increasing Sensitivity Demands Utilizing the Atomx Concentrator/Multimatrix Autosampler
February 01, 2010
Environmental
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In this study, data was collected to evaluate carryover of target analytes in subsequent blanks following high concentration samples.
Determination of Sulfonamide Antibiotics in Bovine Liver Using Agilent SampliQ QuEChERS EN Kits by LC–MS-MS
February 01, 2010
Pharmaceutical
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The procedure involves a rapid and efficient pretreatment with SampliQ QuEChERS kits. The homogenized liver sample was initially extracted in a buffered aqueous/1% acetic acid acetonitrile system with an extraction and partitioning step after the addition of salts.
Mega-Dalton Protein Complexes Characterization
February 01, 2010
Biological
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Recent technological advances in macromolecular crystallography led to focus on the study of more and more sophisticated biological systems, such as protein-protein complexes.
Determination of Quinolone Antibiotics in Bovine Liver Using Agilent SampliQ QuEChERS Kits by LC–MS-MS
February 01, 2010
Pharmaceutical
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The procedure involves a rapid and efficient pretreatment by SampliQ QuEChERS kits. The homogenized liver sample was initially extracted in a buffered aqueous, 5% formic acid acetonitrile system.
Enhanced Peak Capacity for Polyphenols in Wine Using a Longer Agilent ZORBAX RRHD StableBond C18 1.8 µm Column
February 01, 2010
Food and Beverage
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Greater peak capacity and resolution can be easily obtained for gradient analysis of complex samples by using sub-2 µm particles in longer column lengths.
GC–MS Analysis of Volatile Organic Compounds in Drinking Water: Productivity Solution for U.S. EPA Method 524.2
February 01, 2010
Environmental
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The GC–MS analysis of volatile organics in drinking water according to United States Environmental Protection Agency (US EPA) Method 524.2 offers a number of analytical challenges, ranging from water handling to very low target compound concentrations.
Enantioselective Reversed-Phase Chromatography with Protein-based Columns
February 01, 2010
Chiral
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Excellent reversed-phase separations using a protein-based column, CHIRAL-AGP, with volatile LC–MS compatible buffer systems have been obtained for a range of basic solutes.