Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques.
Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques. maXis™ is the only mass spectrometer able to deliver the maximum MS performance specification at the very highest speeds delivered by modern Ultra Performance Liquid Chromatography (UPLC) and Capillary Electrophoresis (CE). The maXis is specially designed to deliver excellent results in many applications including:
With resolution in excess of 40,000 and MS and MS-MS mass accuracy typically between 600–800 ppb at speeds of up to 20 full spectra per s simultaneously. No other mass spectrometer is better equipped to deliver definitive data on complex samples in proteomics, metabolomics, and small molecule identification challenges.
Figure 1: Extracted ion chromatograms of Â±1mDa at acquisition speed of 10 spectra per s analyzed under 85 s.
Typical results from the maXis:
Sample: Club Drug Mix of 10 Small Molecule Drugs
Figure 2: maXisâ¢ and SmartFormulaâ¢ routinely deliver confidence in molecular identification with unsurpassed sub-ppm accuracy.
Waters Acquity UPLC with DAD detector (210–400 nm)
Column: Acquity BEH C18 1.7 μm 2.1 × 50 mm
Column Temperature: 40 °C
A) 0.1% Formic Acid in Water
B) 0.1% Formic Acid in ACN
20%–40% B in 60 s
40%–100% B in 5 s
100% B for 172 s *followed by column equilibration
Flow Rate: 0.6 mL/min
Injection volume: 2 μL
Bruker Daltonics maXis
Spectral Acquisition 10 full spectra per s
Dry Heater 180 °C
Nebulizer 2 Bar
Capillary 4500 V
Dry Gas 6 L/min
ESI positive polarity
Range 100–800 m/z
Calibrant Lithium Formate (16mM)
LockMass Calibration Methylstearate
In this study, the maXis was challenged to identify a series of unknown drug compounds that had been separated by UPLC. Using a typical reverse phase C18 gradient separation method, 7 of the 10 compounds were base line separated by the UPLC in 85 s at 10 Hz sampling rate. The maXis was able to discern the presence of 10 compounds in the sample, despite the lack of chromatographic separation of three of the compounds, even at levels as low as 10 pg on column. Furthermore, the excellent mass accuracy (<1 ppm) and resolution (Avg ~50 K) of the results generated by the maXis, when used in conjunction with the SmartFormula 3D software, were able to correctly identify all 10 compounds and unequivocally determine their molecular formula. This is exceptional performance from a qTOF, especially with a UPLC speed separation.
Bruker Daltonics Inc.
40 Manning Rd., Billerica, MA 01821
tel. (978)663-3660, fax (978)667-5993