The Application Notebook-09-01-2010

September 01, 2010

Cationic surfactants are used as disinfectants, corrosion inhibitors, phase-transfer catalysts, wetting agents, dispersants, and even mobile phase modifiers in liquid chromatography.

September 01, 2010

ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8?C40 aliphatics, C10?C22 aromatics).

September 01, 2010

Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.

September 01, 2010

Cocaine is a central nervous system stimulant derived from Erythroxylon coca.

September 01, 2010

Protein-based pharmaceutical drugs, such as fusion proteins and monoclonal antibodies (mAb), are playing an increasing role in modern therapeutics.

September 01, 2010

Polysaccharide-based chiral stationary phases (CSPs) are often utilized for a broad range of enantiomeric chromatographic separations due to their wide chiral recognition ability.

September 01, 2010

Furan is naturally occurring at low levels in many foods and drinks (1).

September 01, 2010

The new MAbPacTM SCX-10 column is based on 10 µm nonporous, highly cross-linked styrene-type polymeric media with a proprietary uniform hydrophilic coating that is grafted with sulfonic acid functional groups.

September 01, 2010

Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest.

September 01, 2010

The Chirosil RCA(+) and SCA(–) chiral stationary phases (CSPs) are specialized chiral phases very effective in separating various natural and unnatural amino acids as well as compounds containing primary amines.

September 01, 2010

An extension of ASTM D7169 is shown using new Zebron ZB-1XT SimDist metal GC columns.

September 01, 2010

In developing the Atomx automated sample prep system, Teledyne Tekmar integrated both a P&T concentrator and a multi-matrix autosampler into a single platform system that can be used in the analysis of soil, water, and soils requiring methanol extraction.

September 01, 2010

Illicit cocaine, amphetamine, methamphetamine, and ecstasy (MDMA) are often encountered in the form of salts and identification of the specific counter ion present may provide valuable information about the source of production and the manufacturing pathway.

September 01, 2010

Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications.

September 01, 2010

Coffees have aroma and taste based on where the beans are grown, roasting techniques, and flavor additives.

September 01, 2010

This note examines the benefits of using flash or HPLC for compound purification. Results show that flash is a viable alternative to HPLC.

September 01, 2010

Burning mosquito coils to repel these pests is common in many countries; however the emission of various, potentially toxic volatile organic compounds (VOCs) is cause for concern.

September 01, 2010

N-Nitrosodimethylamine and other structurally similar nitrosamines are classified as probable carcinogens by the U.S. EPA, and their presence has been reported in California drinking water since 1998.

September 01, 2010

Levoglucosan is a combustion product of cellulose and, as such, widely used as a unique tracer for biomass burning sources in atmospheric aerosols.

September 01, 2010

Agilent Poroshell 120, a superficially porous particle column, offers resolution and speed similar to columns packed with 1.8-µm totally porous particles, without generating high back pressure.

September 01, 2010

Gel filtration chromatography is the primary method used to analyze the amount of aggregate and dimer present in a therapeutic protein sample.

September 01, 2010

This paper describes the use of the AB SCIEX TripleTOFTM 5600 LC–MS-MS system for targeted and nontargeted screening for pesticide residue in food samples.

September 01, 2010

Fast, flexible platforms for peptide quantification are needed, particularly for a discovery setting.

September 01, 2010

LCGC is planning to publish the next issue of The Application Notebook special supplement in December.

September 01, 2010

The optical isomers of flavanone have been separated using the EpitomizeTM CSP-1 series of chiral stationary phases.

September 01, 2010

This article reports a new sample injection mechanism for an autosampler that directly transfers a sample to column without passing it through any flow-changing device, and hence minimizes a delay volume and reduces a carryover signal to the extreme level.

September 01, 2010

In this application note we present an online SPE-LC method for the determination of trace-level endocrine disruptive compounds (EDCs) in seawater using the new SolEx HRP cartridge as the solid-phase extraction (SPE) phase.

September 01, 2010

A sample of ambient cabin air from a commercial airliner was collected by stir bar sorption extraction (SBSE) and analyzed by thermal desorption GCxGC-TOFMS.

September 01, 2010

To the members of LCGC's readership, the science behind the headlines is always apparent. Separation science in general, and chromatography in particular, find application in nearly every facet of everyday life, from pharmaceutical analysis to the biomedical field to environmental testing, and this Application Notebook is a tribute to just how thoroughly separation science permeates our daily lives.

September 01, 2010

The new MAbPacTM size exclusion (SEC) column is based on high-purity, spherical, porous (300 Å), 5 µm silica covalently modified with a proprietary diol hydrophilic layer.

September 01, 2010

Site-specific analysis of protein glycosylation has become a very important issue in pharmaceutical/biotech industries, requiring specific analytical tools and methodologies.

September 01, 2010

Urine is often used to analyze drugs of abuse because it is easy to collect and is available in copious amounts.

September 01, 2010

Aflatoxins occur naturally in peanuts, cottonseed, corn, and dried chili pepper, as well as many mixed or processed foods and feeds.

September 01, 2010

Method development in reversed-phase liquid chromatography (RPLC) can be challenging for structurally similar compounds.

September 01, 2010

Electrochemical detection (ECD) is both selective and extremely sensitive. However, relatively few compounds are electrochemically active, therefore limiting its application.

September 01, 2010

Complete high resolution cross-fractionation of Polyolefins in the molar mass and composition dimensions is achieved with a new benchtop CFC analytical instrument.

September 01, 2010

Methods: Vials and closures were exposed to acetonitrile for 2 h and subsequently analysed by LC-UV, LC–MS, and GC–MS to characterize the interferences.

September 01, 2010

Whisky is a complex and variable material, the quality of which is affected by the raw materials and processing.

September 01, 2010

Gel-permeation chromatography (GPC) is a highly effective postextraction cleanup method for removing high molecular-weight interferences such as lipids, proteins, and pigments from sample extracts prior to analysis.

September 01, 2010

P-hydroxybenzoic acid esters, also called parabens, are widely used as antimicrobial preservatives in cosmetic products.

September 01, 2010

Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques.

September 01, 2010

The QuEChERS approach is used for the extraction of 16 PAH compounds from fish with analysis by LC using fluorescence detection.

September 01, 2010

Since the addition of electron transfer dissociation (ETD) to the Thermo Scientific LTQ Orbitrap hybrid mass spectrometers, ETD has become a standard tool for proteomics research together with the traditional collision-induced dissociation (CID) (1).

September 01, 2010

For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.

September 01, 2010

Click the title above to open the LCGC North America September 2010 Application Notebook, Vol 28 No s9, in an interactive PDF format.