The Application Notebook-09-01-2010
The Application Notebook
Use of Alternative Solvents for Liquid Chromatographic Analysis of Cationic Surfactants
September 01, 2010
General
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Cationic surfactants are used as disinfectants, corrosion inhibitors, phase-transfer catalysts, wetting agents, dispersants, and even mobile phase modifiers in liquid chromatography.
Fractionation of Extractable Petroleum Hydrocarbons from Soil Using ISOLUTE® EPH SPE Columns
September 01, 2010
Environmental
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ISOLUTE EPH columns have been developed to fractionate pentane or hexane based soil extracts into aliphatic and aromatic hydrocarbon fractions (C8?C40 aliphatics, C10?C22 aromatics).
Virus Particle Characterization
September 01, 2010
Biological
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Viruses are packets of infectious nucleic acid (either DNA or RNA) surrounded by a protective coat consisting of a large number of protein subunits.
Confirmation and Quantitation of Cocaine and Major Metabolites in Urine Using the ISQ Single Quadrupole GC–MS
September 01, 2010
General
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Cocaine is a central nervous system stimulant derived from Erythroxylon coca.
Peptide Mapping of a Monoclonal Antibody Using Poroshell 120
September 01, 2010
Biological
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Protein-based pharmaceutical drugs, such as fusion proteins and monoclonal antibodies (mAb), are playing an increasing role in modern therapeutics.
Achieving Enantiomeric Separation of Anti-Ulcer Drugs Using Lux Cellulose-4
September 01, 2010
Pharmaceutical
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Polysaccharide-based chiral stationary phases (CSPs) are often utilized for a broad range of enantiomeric chromatographic separations due to their wide chiral recognition ability.
Determination of Furan in Food by Gas Chromatography– Mass Spectrometry and Headspace Sampling
September 01, 2010
Food and Beverage
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Furan is naturally occurring at low levels in many foods and drinks (1).
A New High-Resolution MAbPac SCX Column for Characterization of Monoclonal Antibody Heterogeneity
September 01, 2010
Biological
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The new MAbPacTM SCX-10 column is based on 10 µm nonporous, highly cross-linked styrene-type polymeric media with a proprietary uniform hydrophilic coating that is grafted with sulfonic acid functional groups.
Characterization Studies of PEGylated Lysozyme Using TSK-GEL® HPLC Columns
September 01, 2010
Biological
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Chemical modification of therapeutic proteins in order to enhance their biological activity is of increasing interest.
Effects of Various Acids and Acid Concentration on a ChiroSil® Crown-Ether Chiral Stationary Phase
September 01, 2010
Chiral
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The Chirosil RCA(+) and SCA(–) chiral stationary phases (CSPs) are specialized chiral phases very effective in separating various natural and unnatural amino acids as well as compounds containing primary amines.
Extending the Range of High Temperature Simulated Distillation (ASTM D7169) to C120 Using New Zebron ZB-1XT SimDist GC Columns
September 01, 2010
Industrial
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An extension of ASTM D7169 is shown using new Zebron ZB-1XT SimDist metal GC columns.
Calibrating Gasoline Range Organics (GROs) Using an Automated Dilution Scheme with the Atomx Concentrator/Multi-Matrix Autosampler
September 01, 2010
Environmental
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In developing the Atomx automated sample prep system, Teledyne Tekmar integrated both a P&T concentrator and a multi-matrix autosampler into a single platform system that can be used in the analysis of soil, water, and soils requiring methanol extraction.
An Accurate and Robust LC–MS Method for the Identification of Illicit Drug Salt Forms
September 01, 2010
General
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Illicit cocaine, amphetamine, methamphetamine, and ecstasy (MDMA) are often encountered in the form of salts and identification of the specific counter ion present may provide valuable information about the source of production and the manufacturing pathway.
Hydroxyethylstarches (HES)
September 01, 2010
Biological
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Hydroxyethylstarches (HES) are used increasingly as plasma expanders in medical applications.
Determination of Furan in Coffee by Static and Dynamic Headspace-GC–MS
September 01, 2010
Food and Beverage
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Coffees have aroma and taste based on where the beans are grown, roasting techniques, and flavor additives.
Is There Really a Difference Between Flash and HPLC for LC Purification?
September 01, 2010
General
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This note examines the benefits of using flash or HPLC for compound purification. Results show that flash is a viable alternative to HPLC.
Determining the Health Impact of Burning Mosquito Coils Using Versatile Micro-Chamber Sampling and TD-GC–MS
September 01, 2010
Environmental
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Burning mosquito coils to repel these pests is common in many countries; however the emission of various, potentially toxic volatile organic compounds (VOCs) is cause for concern.
Nitrosamines in Drinking Water
September 01, 2010
Environmental
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N-Nitrosodimethylamine and other structurally similar nitrosamines are classified as probable carcinogens by the U.S. EPA, and their presence has been reported in California drinking water since 1998.
Determining Saccharidic Tracers in Atmospheric Aerosols
September 01, 2010
Biological
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Levoglucosan is a combustion product of cellulose and, as such, widely used as a unique tracer for biomass burning sources in atmospheric aerosols.
Fast, Low Pressure Analysis of Food and Beverage Additives Using Agilent Poroshell 120
September 01, 2010
Food and Beverage
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Agilent Poroshell 120, a superficially porous particle column, offers resolution and speed similar to columns packed with 1.8-µm totally porous particles, without generating high back pressure.
Aggregation Analysis of Biosimilar EPO (Erythropoietin) Using a BioSep 2000 GFC Column
September 01, 2010
Biological
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Gel filtration chromatography is the primary method used to analyze the amount of aggregate and dimer present in a therapeutic protein sample.
Pesticide Residues Screening in Food Samples Using High Resolution and Accurate Mass LC–MS-MS
September 01, 2010
Food and Beverage
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This paper describes the use of the AB SCIEX TripleTOFTM 5600 LC–MS-MS system for targeted and nontargeted screening for pesticide residue in food samples.
A Flexible SPE-LC–MS–MS Platform for the Simultaneous Quantification of Multiple Amyloid β Peptides in Cerebrospinal Fluid
September 01, 2010
Biological
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Fast, flexible platforms for peptide quantification are needed, particularly for a discovery setting.
Call for Application Notes
September 01, 2010
Articles
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LCGC is planning to publish the next issue of The Application Notebook special supplement in December.
The Separation of Racemic Flavanone on Epitomize™ CSP-1 Chiral Stationary Phases
September 01, 2010
Chiral
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The optical isomers of flavanone have been separated using the EpitomizeTM CSP-1 series of chiral stationary phases.
Next-Generation Autosampler for LC–MS Equipped with Straight Injection TechnologyTM
September 01, 2010
General
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This article reports a new sample injection mechanism for an autosampler that directly transfers a sample to column without passing it through any flow-changing device, and hence minimizes a delay volume and reduces a carryover signal to the extreme level.
Determination of Trace-Level Endocrine Disruptors in Seawater by Online SPE-HPLC Using the SolEx HRP Cartridge
September 01, 2010
Environmental
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In this application note we present an online SPE-LC method for the determination of trace-level endocrine disruptive compounds (EDCs) in seawater using the new SolEx HRP cartridge as the solid-phase extraction (SPE) phase.
Analysis of Aircraft Cabin Air by TD-GCXGC-TOFMS
September 01, 2010
Environmental
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A sample of ambient cabin air from a commercial airliner was collected by stir bar sorption extraction (SBSE) and analyzed by thermal desorption GCxGC-TOFMS.
The Application Notebook: A More Critical Resource Than Ever
September 01, 2010
From the Publisher
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To the members of LCGC's readership, the science behind the headlines is always apparent. Separation science in general, and chromatography in particular, find application in nearly every facet of everyday life, from pharmaceutical analysis to the biomedical field to environmental testing, and this Application Notebook is a tribute to just how thoroughly separation science permeates our daily lives.
A New High-Resolution Size Exclusion Chromatography Column for MAb Aggregate Analysis
September 01, 2010
Biological
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The new MAbPacTM size exclusion (SEC) column is based on high-purity, spherical, porous (300 Å), 5 µm silica covalently modified with a proprietary diol hydrophilic layer.
Glycosylation Analysis by Dedicated Sample Preparation and Complementary nano-LC (HILIC/RP)/ESI-MS
September 01, 2010
Biological
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Site-specific analysis of protein glycosylation has become a very important issue in pharmaceutical/biotech industries, requiring specific analytical tools and methodologies.
Analyzing the SAMHSA Drug Panel on Kinetex Core-Shell Media
September 01, 2010
General
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Urine is often used to analyze drugs of abuse because it is easy to collect and is available in copious amounts.
Analysis of Aflatoxins in Ground Peanuts and Peanut Butter by HPLC with Photochemical Derivatization
September 01, 2010
Food and Beverage
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Aflatoxins occur naturally in peanuts, cottonseed, corn, and dried chili pepper, as well as many mixed or processed foods and feeds.
Orthogonal Selectivity of ZirChrom®-CARB
September 01, 2010
General
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Method development in reversed-phase liquid chromatography (RPLC) can be challenging for structurally similar compounds.
Making Non-Electrochemically Active Compounds Electrochemically Active
September 01, 2010
Environmental
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Electrochemical detection (ECD) is both selective and extremely sensitive. However, relatively few compounds are electrochemically active, therefore limiting its application.
Microstructure Characterization of Polyolefins by Automated Cross-Fractionation Chromatography (CFC)
September 01, 2010
Polymer
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Complete high resolution cross-fractionation of Polyolefins in the molar mass and composition dimensions is achieved with a new benchtop CFC analytical instrument.
How Clean Are Your Vials and Closures?
September 01, 2010
General
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Methods: Vials and closures were exposed to acetonitrile for 2 h and subsequently analysed by LC-UV, LC–MS, and GC–MS to characterize the interferences.
GC Column Selection for the Analysis of Volatiles and Higher Alcohols in Whisky
September 01, 2010
Food and Beverage
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Whisky is a complex and variable material, the quality of which is affected by the raw materials and processing.
GPC Clean-Up of Black Pepper Prior to Analysis for Organochlorine (OC) Pesticides by GC/XSD
September 01, 2010
Environmental
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Gel-permeation chromatography (GPC) is a highly effective postextraction cleanup method for removing high molecular-weight interferences such as lipids, proteins, and pigments from sample extracts prior to analysis.
Rapid UHPLC Determination of Common Preservatives in Cosmetic Products
September 01, 2010
General
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P-hydroxybenzoic acid esters, also called parabens, are widely used as antimicrobial preservatives in cosmetic products.
High Resolution Analysis of an Intact Antibody and Its Component Light Chains
September 01, 2010
Biological
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Modern analytical applications often demand definitive tandem MS results on ever more complex samples utilizing fast separation techniques.
Extraction of Polycyclic Aromatic Hydrocarbons (PAH) from Fish Using the QuEChERS Approach*
September 01, 2010
Food and Beverage
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The QuEChERS approach is used for the extraction of 16 PAH compounds from fish with analysis by LC using fluorescence detection.
Increasing Proteome Coverage with ETD and CID in One Run Using Intelligent Data-Dependent Decision Tree Logic
September 01, 2010
Biological
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Since the addition of electron transfer dissociation (ETD) to the Thermo Scientific LTQ Orbitrap hybrid mass spectrometers, ETD has become a standard tool for proteomics research together with the traditional collision-induced dissociation (CID) (1).
Extend UHPLC Column Lifetime with KrudKatcherTM Ultra In-Line Filters
September 01, 2010
General
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For many years, the use of guard columns has been advocated by column manufacturers and other experts to protect and extend column lifetime and performance of analytical columns from potential damage caused by the presence of chemical contaminants and microparticulates in the sample and mobile phase.
Vol 28 No 9 LCGC North America September 2010 Application Notebook PDF
September 01, 2010
Issue PDF
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Click the title above to open the LCGC North America September 2010 Application Notebook, Vol 28 No s9, in an interactive PDF format.