The Column-12-15-2014

The Column

Analysis of Monoclonal Antibodies and Their Fragments by SEC Coupled with HRAM-MS

December 15, 2014

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The biopharmaceutical industry continues to focus on the development of biotherapeutic monoclonal antibody (mAbs) drugs. In this article, the compatibility of SEC coupled with HRAM–MS for the analysis of mAbs is demonstrated.

Application of Silica Monoliths for Bioanalysis

December 15, 2014

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In this article, the application of silica monoliths with bimodal pore structure (macro and mesopores) is described for the chromatographic analysis of biomolecules including peptides, proteins, and antibodies.

Vol 10 No 22 The Column December 15, 2014 North American PDF

December 15, 2014

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Click the title above to open The Column December 15, 2014 North American issue, Volume 10, Number 22, in an interactive PDF format.

Vol 10 No 22 The Column December 15, 2014 Europe and Asia PDF

December 15, 2014

Issue PDF

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Click the title above to open The Column December 15, 2014 Europe & Asia issue, Volume 10, Number 22, in an interactive PDF format.

Detecting Nerve Agent Exposure

December 14, 2014

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Scientists at the Norwegian Defence Research Establishment (Kjeller, Norway) are working in collaboration with the University of Oslo (Oslo, Norway) to develop new methods to detect the illicit use of nerve agents. In a recent study published in the journal Analytical Chemistry, the team presented a novel method for the quantification of nerve agent metabolites in human serum and urine by combining salting-out assisted liquid–liquid extraction (SALLE) and on-line solid-phase extraction–liquid chromatography–tandem mass spectrometry (SPE–LC–MS–MS).

Metabolic Profiling of Tea

December 14, 2014

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A new study published in the Journal of Chromatography A presents metabolite profiling of tea (Camella sinensis) harvested from the Bulang Mountains in Yunnan, China, using multidimensional gas chromatography–mass spectrometry (GC×GC–MS) to determine the impact of seasonal changes.

Speeding Up Drug Metabolism Screening

December 14, 2014

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A new study published in the journal Analytical Chemistry presents a drug metabolism strategy based on microsome mesoporous organosilica nanoreactors coupled with high performance liquid chromatography–mass spectrometry (HPLC–MS) to screen for potential drug toxicity.

Instrumental Innovations 2014

December 14, 2014

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Selected highlights of innovative chromatography products.