CHROMacademy Training Debuts in Africa
The 3rd African Liquid Chromatography Workshop was held on 6–10 October 2014 in Nairobi, Kenya, at the ChromAfrica Training Centre.
The 3rd African Liquid Chromatography Workshop was held on 6–10 October 2014 in Nairobi, Kenya, at the ChromAfrica Training Centre. The workshop featured five days of lectures and laboratory practicals on high performance liquid chromatography (HPLC) method development, column selection, and sample preparation. Merlin Bicking of ACCTA in Minnesota, USA, delivered training to 10 students from both government and private laboratories. Columns were supplied by Supelco and Agilent Technologies for use in the demonstrations. On-line CHROMacademy sections on sample preparation and solid-phase extraction (SPE) were an important part of the training.
www.chromacademy.com/CHROMacademy-in-Africa.html
Influence of Concentration in Conventional GPC/SEC and Advanced Detection GPC/SEC
March 21st 2025Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
