The Column-12-08-2020

Incognito leads us through the complex method used to determine related substances for hydroxychloroquine, exploring the practical implementation and critical evaluation of a less than optimum ultrahigh-pressure liquid chromatography (UHPLC) pharmacopeial method.

Field-flow fractionation (FFF) coupled to light scattering is a powerful method to separate and characterize nanoparticles, proteins, and polymers from a few nanometres to a few micrometres. The technique is one of the few that can cover the full size range of nanomaterials and provide high-resolution size distributions and additional characterization. New developments in FFF enhance performance and productivity.

Here are 10 great tips for optimizing LC-Electrospray Ionization (ESI)-MS so that you achieve the best possible results every time. These tips and tricks have been collated by my colleagues to form a beginner’s guide to LC-(ESI)MS and as a primer for those who are already using the technique.