Sample Preparation Perspectives
During the developmental stages of chromatography, the use of sample derivatization was prevalent either to render difficult-to-separate analytes ready for chromatography or to improve analyte detectability. As chromatography techniques advanced, including detection, the need for derivatization was less pronounced. Today, there is a resurgence of interest in derivatization schemes; however, the new generation of separation scientists is not as well-versed in these reactions. Some of this resurgence is for newer purposes, such as combining two or more derivatizations for the analysis of multiple compound classes, or to develop greener approaches. In this month’s instalment, we explain the use of chemical derivatization in the separation sciences, generally, and in sample preparation specifically.