LCGC North America-03-01-2015

One of the most frequent times that we discover a problem with a liquid chromatography (LC) method is when we examine a data set following the analysis of a batch of samples.

 There has been a revival of supercritical fluid chromatography (SFC) in recent years, especially in the chiral preparative field, but also more recently in the analytical area. However, SFC is considerably more complex than liquid chromatography (LC), mainly because of the compressibility of the mobile phase. One can say that SFC is a "rubber variant" of LC where everything considered constant in LC varies in SFC. In this review, we go through advances in theory, instrumentation, and novel applications.

The transit of peaks through a gas chromatography (GC) column depends strongly on the thermal profile they encounter on the way.

Limonin was separated from other components in citrus juice sample matrices by reversed-phase chromatography with ultraviolet detection.

LCGC recently spoke to Miguel Herrero of the Institute of Food Science Research (CIAL-CSIC) at the Spanish National Research Council, in Madrid, Spain, about his research in foodomics-based approaches, the evolution of food analysis, and the benefits of two-dimensional (2D) liquid chromatography (LC) in this application.

There is a bewildering array of stationary-phase choices available for reversed-phase high performance liquid chromatography (HPLC), and even within each phase designation (such as "C18") the selectivity of each phase can vary widely.

Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation?

HIGHLIGHTS OF APPS FOR SEPARATION SCIENCE

Click the title above to open the LCGC North America March 2015 regular issue, Vol 33 No 3, in an interactive PDF format.