In his article, published in 2000, David V. McCalley found large increases in the efficiency for basic compounds at elevated temperature
Despite the utility of high performance liquid chromatography (HPLC) simulators, we found that all the free and low-cost simulators were outdated or had extremely limited functionality, so we created one that addressed these shortcomings. We developed a sophisticated, open-source HPLC simulator that is available for free as well as a version for Android users called "HPLC Simulator." Here we discuss a few questions that the simulator can help answer: Why are peaks narrower in gradient elution? What is gradient delay and how does it affect a separation?
The guest authors describe an optimization procedure to achieve the highest plate count in a given analysis time.
Well-known industry veteran and LCGC EAB member Peter Carr looks back at the early days of the chromatography field and reflects on his own experiences.
We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model
We focus on the characterization of reversed-phase columns with particular emphasis on the hydrophobic-subtraction model